Abstract
A high-performance liquid chromatography-electrospray ionization-mass spectromentry (LC–ESI–MS) method has been developed for the determination of picroside II in dog plasma. Plasma samples were deproteinated with acetonitrile and a Hypersil ODS2 column was used with a mobile phase consisted of methanol-water. The determination was validated in the concentration range of 0.10–50 μg mL−1 using 50 μL of plasma. The method was successfully applied to a pharmacokinetic study of picroside II.
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Acknowledgments
This project was supported by the National Natural Science Foundation of China (No. 30472060).
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Yu, QL., Zhao, Y., Chen, XJ. et al. A Sensitive LC–ESI–MS Method for Pharmacokinetic Studies of Picroside II in Dog Plasma. Chroma 67, 1013–1016 (2008). https://doi.org/10.1365/s10337-008-0591-9
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DOI: https://doi.org/10.1365/s10337-008-0591-9