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Impact of Flavonoid Composition of Medicinal Plants: Difficulties in Selecting an LC Method

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Abstract

In order to understand the role of flavonoids in the pharmacodynamics of medicinal plants the impact of flavonoid composition on antioxidant activity was studied. Antioxidant capacity was determined by TEAC assay, flavonoid composition was examined by liquid chromatography. This study presents the difficulties experienced in selecting a proper method for analyzing flavonoid aglycones. Standard molecules and plant extracts were examined applying three HPLC systems. System 1 (RP-C18, ACN, CH3COOH-H2O) is similar to the one formerly used for flavonoid glycosides, System 2 (HS-PEG, MeOH-HCOOH, HCOOH-H2O) was recommended by the manufacturer for the very purpose, System 3 (RP-C18, MeOH, H3PO4-H2O) was developed in our laboratory. Our results show that owing to the complexity of plant extracts, certain identification can only be attained by analyzing the samples in at least three chromatographic systems. Otherwise overlapping of bands may lead to misinterpretation of the chromatograms.

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Authors and Affiliations

  1. Department of Pharmacognosy, Semmelweis University, Üllöi Street 26, 1085, Budapest, Hungary

    V. Vukics, B. Hevesi T, Á. Fukász & Á. Kéry

Authors
  1. V. Vukics
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  2. B. Hevesi T
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  3. Á. Fukász
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  4. Á. Kéry
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Corresponding author

Correspondence to V. Vukics.

Additional information

Presented at: 6th Balaton Symposium on High-Performance Separation Methods, Siófok, Hungary, September 7–9, 2005

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Vukics, V., Hevesi T, B., Fukász, Á. et al. Impact of Flavonoid Composition of Medicinal Plants: Difficulties in Selecting an LC Method. Chroma 63 (Suppl 13), S125–S129 (2006). https://doi.org/10.1365/s10337-006-0727-8

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  • DOI: https://doi.org/10.1365/s10337-006-0727-8

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