Abstract
Four naphthoquinones namely 7-methyljuglone, diospyrin, neodiospyrin and isodiospyrin present in Euclea natalensis subsp. natalensis (Ebenaceae), have been found to have potent antituberculosis activity. A comparative study of these naphthoquinones was conducted in the different species of Euclea found in South Africa, in order to select a plant species with the highest concentration of naphthoquinones for preclinical studies. High performance liquid chromatography was used to quantify the concentration of each naphthoquinone. Isodiospyrin showed the best interday values with a relative error of 2.4% and diospyrin the lowest reproducibility with a relative error value of 13%. The intraday determination showed that isodiospyrin had the lowest reproducibility with a relative error of 13.6%, in contrast to 7-methyljuglone which exhibited satisfactory reproducibility with a relative error of 4.64%. The most potent antituberculosis compound, 7-methyljuglone was found in almost all species studied and the concentration ranged between 20 and 1,300 mg kg−1. The concentration of 7-methyljuglone was found to be the highest in E. pseudebenus. Of all the plants studied, only one of the E. pseudebenus specimens showed the presence of all four compounds in significant quantities. Variation observed with regard to the concentration of the four compounds in the different Euclea species could be due to the age of the roots harvested, wound, and environmental and/or other stress factors.
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References
Van Wyk B-E, Gericke N (2000) People’s plants: a guide to useful plants of Southern Africa. Briza publications, Pretoria
Van Wyk B, Van Wyk P (1997) Field guide to trees of southern Africa. Struik Publishers, Cape Town
Schiafella F, Fratini L, Mezzetti T, Bellavita V (1975) Phytochemistry 14:584–585
Áurea Cruz Costa M, Lopes MH, Margarida IP, Ferreira MA, Alves AC (1976) Phytochemistry 15:829
Van der Vijver LM, Gerritsma KW (1973) Phytochemistry 12:230–231
Ferreira MA, Áurea Cruz Costa M, Alves A, Lopes MH (1974) Phytochemistry 13:1587–1589
Van der Vijver LM, Gerritsma KW (1974) Phytochemistry 13:2322–2323
Tannock J (1973) Phytochemistry 12:2066–2067
Lall N, Das Sarma M, Hazra B, Meyer JJM (2003) J Antimicrob Chemother 51:435–438
Magama S, Pretorius JC, Zietsman PC (2003) S Afr J Bot 69:193–198
Lall N, Meyer JJM. (2000) J Ethnopharmacol 72:313–316
De Moura KCG, Emery FS, Neves-Pinto C, Pinto MFR, Dantas AP, Salomao K, De Castro SL, Pinto AV (2001) J Braz Chem Soc 12:325–338
Lall N, Meyer JJM (2001) J Ethnopharmacol 78:213–216
Charkrabarty S, Roy M, Hazra B, Bhattacharya RK (2002) Can Lett 188:85–93
Hazra B, Kumar B, Biswas S, Pandey BN, Mishra KP (2005) Toxicol Lett 157:109–117
Ting C-Y, Hsu C-T, Hsu H-T, Su J-S, Chen T-Y, Tarn W-Y, Kuo Y-H, Whang-Peng J, Lui LF, Hwang J (2003) Biochem Pharmacol 66:1981–1991
Van der Kooy F, Meyer JJM, Lall N (2006) S Afr J Bot 72:349–352
Sanyal U, Bhattacharyya S, Patra A, Hazra B (2003) J Chromatogr A 1017:225–232
Close CD, McArthur C (2002) OIKOS 99:166–172
Khan MR, Matusa SL, Ndaalio G, Wevers H (1978) Pakistan J Sci Ind Res 21:197–199
Gupta MM, Verma RK, Uniyal GC, Jain SP (1993) J Chromatogr A 637:209–212
Van der Vijver LM, Gerritsma KW (1975) J Chromatogr A 114:443–450
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Joubert, A., van der Kooy, F., Meyer, J.J.M. et al. HPLC in the Comparative Study of the Content of Naphthoquinones (Quinonoid Constituents) in Euclea Species of South Africa. Chroma 64, 399–403 (2006). https://doi.org/10.1365/s10337-006-0055-z
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DOI: https://doi.org/10.1365/s10337-006-0055-z