Abstract
A high-performance liquid chromatographic method with photodiode array detection has been developed for the determination of squalene. After treated by extraction and fractional crystallization, squalene was analyzed on a C18 column (150 × 3.9 mm, 5 μm) with acetonitrile as mobile phase. Excellent linearity of the calibration curve was observed in the range of 100–40000 μgL−1 and the detection limit was 40 μgL−1. The recoveries were from 89.6% to 100.5% and the relative standard deviations were from 0.5% to 1.4%. The method was successfully applied to the determination of squalene in squalene capsules, olive oil, algal lipids and algal cells.
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Lu, HT., Jiang, Y. & Chen, F. Determination of Squalene Using High-Performance Liquid Chromatography with Diode Array Detection. Chromatographia 59, 367–371 (2004). https://doi.org/10.1365/s10337-003-0176-6
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DOI: https://doi.org/10.1365/s10337-003-0176-6