Multiple-discriminant analysis for light-scattering spectroscopy and imaging of two-layered tissue phantoms

Kazuhiro Gono; Makoto Igarashi; Takashi Obi; Masahiro Yamaguchi; Nagaaki Ohyama

doi:10.1364/OL.29.000971

Optics Letters
Vol. 29,
Issue 9,
pp. 971-973
(2004)
•https://doi.org/10.1364/OL.29.000971

Multiple-discriminant analysis for light-scattering spectroscopy and imaging of two-layered tissue phantoms

Kazuhiro Gono, Makoto Igarashi, Takashi Obi, Masahiro Yamaguchi, and Nagaaki Ohyama

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Kazuhiro Gono, Makoto Igarashi, Takashi Obi, Masahiro Yamaguchi, and Nagaaki Ohyama, "Multiple-discriminant analysis for light-scattering spectroscopy and imaging of two-layered tissue phantoms," Opt. Lett. 29, 971-973 (2004)

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Abstract

We propose a new method for enhancing the sensitivity of the reflectance spectrum to the scattering feature of the superficial tissue layer. This method is based on multiple-discriminant analysis in the eigensubspace of the spectrum. Considering the application of scattering imaging, we evaluated this method by performing multispectral imaging of two-layered tissue phantoms. A color map converted from the spectral reflectance corresponds well to variations in the size of the scattering in the first layer.

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Label	Average ( $µ m$ )	Standard ( $µ m$ )	$μ_{s}^{'}$ ( ${cm}^{- 1}$ )	Number of Density ( ${cm}^{- 3}$ )
03-1	3.52	1.43	3.57	$2.12 \times 10^{8}$
03-2	3.52	1.43	7.13	$1.15 \times 10^{8}$
05-1	4.88	0.84	5.50	$2.11 \times 10^{8}$
05-2	4.88	0.84	10.98	$1.14 \times 10^{8}$
08-1	7.75	0.94	10.63	$2.10 \times 10^{8}$
08-2	7.75	0.94	21.26	$1.05 \times 10^{8}$
10-1	9.3	1.49	15.12	$2.11 \times 10^{8}$
10-2	9.3	1.49	30.24	$1.05 \times 10^{8}$

Label	$μ_{s}^{'} ({cm}^{- 1})$	$μ_{a} ({cm}^{- 1})$
A	7.1	4.5
B	3.5	2.5
C	7.1	4.5
D	3.5	2.5

Label	Average ( $µ m$ )	Standard ( $µ m$ )	$μ_{s}^{'}$ ( ${cm}^{- 1}$ )	Number of Density ( ${cm}^{- 3}$ )
03-1	3.52	1.43	3.57	$2.12 \times 10^{8}$
03-2	3.52	1.43	7.13	$1.15 \times 10^{8}$
05-1	4.88	0.84	5.50	$2.11 \times 10^{8}$
05-2	4.88	0.84	10.98	$1.14 \times 10^{8}$
08-1	7.75	0.94	10.63	$2.10 \times 10^{8}$
08-2	7.75	0.94	21.26	$1.05 \times 10^{8}$
10-1	9.3	1.49	15.12	$2.11 \times 10^{8}$
10-2	9.3	1.49	30.24	$1.05 \times 10^{8}$

Label	$μ_{s}^{'} ({cm}^{- 1})$	$μ_{a} ({cm}^{- 1})$
A	7.1	4.5
B	3.5	2.5
C	7.1	4.5
D	3.5	2.5

Abstract

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Equations (3)

Optics Letters