Data availability

Data acquisition was performed at the MRic Microscopy Rennes Imaging Center (France) with a confocal (Zeiss LSM 880) with airyscan microscope. The argoslide is the Argo-Z slide SLG-121 (Argolight S.A.). Objective used is the Plan-Apochromat 63${\times }$ × /1.4 Oil DIC M27. For excitation of the slide, the 488 laser beam was used with a BP 495-550 + LP 570 emission filter set. ERM-1 (aka ezrin in mammals) is an actin crosslinker enriched in the microvilli that has been endogenously tagged with Neongreen fluorophore by CRISPR-Cas9 [9]. The localization of ERM-1 in microvilli patterns in worms has been characterized in Ref. [10]. The software can process 2D and 3D images. A python library and a napari plugin can be found at [11]. The results presented in this Letter may be obtained from the authors upon reasonable request.