Isolation,Identification of Trichoderma ghanense and Optimization of Spores ProductionChinese Full Text

GAO Xue-li;WU Jian-ping;XU Gang;YANG Li-rong;Zhejiang University,Department of Chemical and Biological Engineering;

Abstract: A strain,having strong antagonistic activity toward phytopathogens,was identified as Trichoderma ghanense by the methods of molecular biology. This strain of Trichoderma ghanense as testing strain,optimization of the solid state fermentation medium was performed to improve the spore yield. Through the single factor experiment,Plackett-Burman experiment,the steepest ascent experiment and the response surface analysis, sugar,corn steep liquor and MgSO4were identified as the most important factors to influence the spore yield. The optimal concentration of the three factors is 0. 56%,0. 56% and 2. 59% respectively. The optimal initial water content of the medium was identified as 35% and the optimal initial pHwas identified as 9. The highest spore yield have been achieved as 1. 16 × 109CFU / g initial dry substrate（ IDS） on the optimized solid state medium, which is 17 times higher than the initial medium.

Keywords：

Trichoderma ghanense Isolation Identification Solid state fermentation Response surface analysis;

DOI：
10.13523/j.cb.20140214
Series：
Subject：
Classification Code：
S476.12

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Isolation,Identification of Trichoderma ghanense and Optimization of Spores ProductionChinese Full Text

GAO Xue-li;WU Jian-ping;XU Gang;YANG Li-rong;Zhejiang University,Department of Chemical and Biological Engineering;

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