Chlorogenic acid is a polyphenol compound, derived from several fruit and plants. The aim of this study was to assess the in vitro antifungal activity of chlorogenic acid and its mode of action. The results indicate that chlorogenic acid exhibits antifungal activities against certain pathogenic fungi in an energy-independent manner, without any hemolytic effect on human erythrocytes. To elucidate the antifungal mode of action of chlorogenic acid, flow cytometry analysis by using DiBAC4(3) and changes in membrane dynamics using 1,6-diphenyl-1,3,5-hexatriene (DPH) were performed with Candida albicans. The results suggest that chlorogenic acid may exert antifungal activity by disrupting the structure of the cell membrane. It is demonstrated that chlorogenic acid is a valid lead compound for the development of bioactive alternatives for treatment of fungal infections.
Conference
International Biotechnology Symposium (IBS 2008): "Biotechnology for the Sustainability of Human Society", 13th, Dalian, China, 2008-10-12–2008-10-17
References
1 10.1016/S1130-1406(08)70027-5, A. Espinel-Ingroff. Rev. Iberoam. Micol.25, 101 (2008).Search in Google Scholar
2 M. R. Jacob, L. A. Walker. Methods Mol. Med.118, 83 (2005).Search in Google Scholar
3 10.1038/35081178, R. A. Dixon. Nature411, 843 (2001).Search in Google Scholar
4 10.1021/jf062614e, S. Fattouch, P Caboni, V. Coroneo, C. I. Tuberoso, A. Angioni, S. Dessi, N. Marzouki, P. Cabras. J. Agric. Food Chem.55, 963 (2007).Search in Google Scholar
5 T. Namba, M. Kurokawa, S. Kadota, K. Shiraki. Yakugaku Zasshi118, 383 (1998).10.1248/yakushi1947.118.9_383Search in Google Scholar
6 10.1016/j.jss.2006.05.037, L. Golkar, X. Z. Ding, M. B. Ujiki, M. R. Salabat, D. L. Kelly, D. Scholtens, A. J. Fought, D. J. Bentrem, M. S. Talamonti, R. H. Bell, T. E. Adrian. J. Surg. Res.138, 163 (2007).Search in Google Scholar
7 10.1021/jf048701t, A. Farah, T. de Paulis, L. C. Trugo, P. R. Martin. J. Agric. Food Chem.53, 1505 (2005).Search in Google Scholar
8 10.1021/jf048270e, S. R. Kanatt, R. Chander, P. Radhakrishna, A. Sharma. J. Agric. Food Chem.53, 1499 (2005).Search in Google Scholar
9 H. Kasai, S. Fukada, Z. Yamaizumi, S. Sugie, H. Mori. Food Chem. Toxicol.38, 467 (2000).Search in Google Scholar
10 10.1271/bbb.63.1295, H. Shibata, Y. Sakamoto, M. Oka, Y. Kono. Biosci. Biotechnol. Biochem.63, 1295 (1999).Search in Google Scholar
11 W. S. Sung, I.-S. Lee, D. G. Lee. J. Microbiol. Biotechnol.17, 1797 (2007).Search in Google Scholar
12 D. G. Lee, Y. Park, P. I. Kim, H. G. Jeong, E. R. Woo, K.-S. Hahm. Biochem. Biophys. Res. Commun.297, 885 (2002).Search in Google Scholar
13 R. S. Liao, R. P. Rennie, J. A. Talbot. Antimicrob. Agents Chemother.43, 1034 (1999).Search in Google Scholar
14 10.1016/S0165-6147(96)01012-7, S. Hartsel, J. Bolard. Trends Pharmacol. Sci.17, 445 (1996).Search in Google Scholar
15 10.1128/AAC.44.10.2873-2875.2000, N. McLain, R. Ascanio, C. Baker, R. A. Strohaver, J. W. Dolan. Antimicrob. Agents Chemother.44, 2873 (2000).Search in Google Scholar PubMed PubMed Central
16 D. F. Wilson, B. Chance. Biochem. Biophys. Acta131, 421 (1967).10.1016/0005-2728(67)90002-3Search in Google Scholar
17 10.1056/NEJM198408093110602, R. S. Klein, C. A. Harris, C. B. Small, B. Moll, M. Lesser, G. H. Friedland. N. Engl. J. Med.311, 354 (1984).Search in Google Scholar PubMed
18 R. S. Liao, R. P. Rennie, J. A. Talbot. Antimicrob. Agents Chemother.43, 1034 (1999).Search in Google Scholar
19 10.1016/j.bbagen.2004.03.005, M. Vincent, L. S. England, J. T. Trevors. Biochim. Biophys. Acta-Gen. Subj.1672, 131 (2004).Search in Google Scholar PubMed
20 F. J. Alvarez-Peral, O. Zaragoza, Y. Pedreno, J. Argüelles. Microbiology148, 2599 (2002).10.1099/00221287-148-8-2599Search in Google Scholar PubMed
21 10.1093/glycob/cwg047, A. D. Elbein, Y. T. Pan, I. Pastuszak, D. Carroll. Glycobiology13, 17R (2003).Search in Google Scholar PubMed
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