Regulation of Quercetin on Expression of TGF-β1 Singal Transduction Pathway in Lung of Silicotic RatsChinese Full Text
PENG Hai-bing;ZHANG Na;WANG Jian-xing;ZHAO Xia;LIU Yan;TIAN Jia-li;WANG Xian-hua;Jitang College,Hebei United University;College of Basic Medical Sciences,Hebei United University;
Abstract: [Objective] To explore the regulation role of quercetin on the expression of transforming growth factor beta 1（TGF-β1） signal transduction pathway in the lung of silicotic rats. [Methods] Totally 75 specific-pathogen-free（SPF） male Wistar rats were randomly divided into three groups: a control group, a model group, and a quercetin group. The rat silicosis model was induced by intratracheal instillation of silicosis（250 mg/kg）, the quercetin group was treated with 1.0 mL saline containg 50 mg/kg quercetin after injection of SiO2, while the rats in the control group were given saline in the same condition. The rats in all groups were put to death on the 3th, 7th, 14th, 21th, 28th days after the silicosis model established. The content of total collagen in lung tissue was determined by hydroxyproline kits. The expression of TGF-β1 protein and p-Smad3/7 in lung tissue samples were evaluated by immunohistochemistry and Western blot technique. [Results] The contents of total collagen in the model group were 6.28, 13.11, 11.59 times of the values in the controls in the same periods respectively（P ＜ 0.01）. The contents of total collagen in the quercetin group were 43.05%, 38.92%, and 37.40% of the corresponding values in the model group in the same periods respectively（P ＜ 0.01）. The expression levels of TGF-β1 were lower in the quercetin group than in the model group in the same periods and higher than in the control group（P ＜ 0.01 and P ＜ 0.05, respectively）. The expression levels of p-Smad3 were higher in the model group than in the quercetin group and in the control group in the same periods（P ＜ 0.01）, but were lower in the quercetin group than in the model group and higher than in the control group in the same periods（P ＜ 0.01）. The expression levels of p-Smad7 were lower in the model group than in the control group and in the quercetin group in the same periods（P ＜ 0.01）, but were higher in the quercetin group than in the model group or in the control group at each designed time spot（P ＜ 0.01）. [Conclusion] Quercetin could decrease the expression of Smad3, increase the expression of Smad7, and then inhibit the collagen protein in silicotic rats.
Keywords：
quercetin; silicosis fi brosis; collagen protein; transforming growth factor beta 1; p-Smad3/7; rats;
- DOI：
10.13213/j.cnki.jeom.2014.0028
- Series：
- Subject：
- Classification Code：
R135.2
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