5-HTT antibody | knockout validation | Frontier Institute HTT-GP-Af1400
DOI
//dx.doi.org/10.13070/ko.en.6.1633
Date
2016-09-24

This is a knockout-validated antibody summary, based on the publication "Physical Interactions and Functional Relationships of Neuroligin 2 and Midbrain Serotonin Transporters", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

5-HTT antibody | knockout validation | Frontier Institute HTT-GP-Af1400 figure 1
Figure 1. Western blots of SERT protein in SERT null mice (KO), SERT heterozygous (HET) and their WT littermates in midbrain (upper panel) or hippocampus (lower panel). Actin is used as loading control, from figure 4 [1].
Antibody information

Antibody: 5-HTT

Catalog number: HTT-GP-Af1400

Summary: Guinea pig polyclonal raised against mouse HTT, 1-77 aa (AF013604). Reacts with mouse and can be used for immunoblot and immunohistochemistry

Company: Frontier Institute

Validation Method

Western Blot

Sample

Freshly dissected brain tissues, homogenization in RIPA buffer (50 mM Tris, pH 7.4, 150 mM NaCl, 1 mM EDTA, 1% TRITON X-100, 1% sodium deoxycholate, 0.1% SDS) containing protease inhibitors (P8340, 1:100; Sigma, St. Louis, MO, USA).

Primary incubation

1:2,000

Detection

chemiluminescence (Western Lightning ECL Pro, Perkin Elmer) and X-ray film (Biomax MS, Kodak, 8294985) exposure.

References
  1. Ye R, Quinlan M, Iwamoto H, Wu H, Green N, Jetter C, et al. Physical Interactions and Functional Relationships of Neuroligin 2 and Midbrain Serotonin Transporters. Front Synaptic Neurosci. 2015;7:20 pubmed publisher