We studied the cell supply system for automation of the cell cloning work. Our aim of this system is the efficient and stable supply some type of cells, egg cells or fibroblasts, to microfluidic chips.In this report, we present the design of air valve system and branch shape for cell transport. For valve system, we made two types of microfluidic chips, one is two layer chip and another is three layer chip. The pneumatic pressure valves were applied in or onto chips, blocked up the flow channel with each method, and changed flow passes.