Promoting Effects of Streptozotocin-induced Diabetes on Induction of Hepatic Preneoplastic Lesions by Diethylnitrosamine in Rats

Kazuhito Ichikawa; Yoshiaki Tagawa; Masaomi Kawai; Makoto Asamoto; Tomoyuki Shirai

doi:10.1293/tox.23.125

Originals

Promoting Effects of Streptozotocin-induced Diabetes on Induction of Hepatic Preneoplastic Lesions by Diethylnitrosamine in Rats

Kazuhito Ichikawa, Yoshiaki Tagawa, Masaomi Kawai, Makoto Asamoto, Tomoyuki Shirai

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Keywords: streptozotocin, diabetes, diethylnitrosamine, liver, GST-P positive foci, hepatocarcinogenesis

JOURNAL FREE ACCESS

2010 Volume 23 Issue 3 Pages 125-131

DOI https://doi.org/10.1293/tox.23.125

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Abstract

The effects of streptozotocin (STZ)-induced diabetes on induction of hepatic preneoplastic lesions by diethylnitrosamine (DEN) were investigated in male Fischer rats. A single dose of STZ was injected intravenously either 2 weeks before or after initiation with DEN. The blood glucose levels were significantly elevated from 1 week after STZ-injection until autopsy. The numbers of GST-P positive foci at 1 week after DEN administration in the STZ-injected rats were similar to those in the non-diabetic rats. In contrast, both the numbers and areas of GST-P positive foci > 2 mm in diameter 8 weeks after DEN administration were increased significantly in the rats treated with STZ after DEN exposure compared with the non-diabetic control rats. The results suggest that hepatic preneoplastic lesions initiated with DEN are promoted by STZ treatment-inducing diabetes.

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