Automated Ribotyping, a Rapid Typing Method for Analysis of Erysipelothrix spp. Strains

Alexandre Tomomitsu OKATANI; Motoko ISHIKAWA; Shin-ichiro YOSHIDA; Michiko SEKIGUCHI; Kenji TANNO; Masuo OGAWA; Tetsuya HORIKITA; Tomoko HORISAKA; Takahide TANIGUCHI; Yukio KATO; Hideki HAYASHIDANI

doi:10.1292/jvms.66.729

NOTE

Automated Ribotyping, a Rapid Typing Method for Analysis of Erysipelothrix spp. Strains

Alexandre Tomomitsu OKATANI, Motoko ISHIKAWA, Shin-ichiro YOSHIDA, Michiko SEKIGUCHI, Kenji TANNO, Masuo OGAWA, Tetsuya HORIKITA, Tomoko HORISAKA, Takahide TANIGUCHI, Yukio KATO, Hideki HAYASHIDANI

Author information

Alexandre Tomomitsu OKATANI
Laboratory of Animal Hygiene, Department of Veterinary Medicine, Faculty of Agriculture, Tokyo University of Agriculture and Technology
Department of Veterinary Public Health II, School of Veterinary Medicine, Azabu University
Motoko ISHIKAWA
Japan Food Research Laboratories, Tama Laboratory
Shin-ichiro YOSHIDA
Japan Food Research Laboratories, Tama Laboratory
Michiko SEKIGUCHI
Japan Food Research Laboratories, Tama Laboratory
Kenji TANNO
Japan Food Research Laboratories, Tama Laboratory
Masuo OGAWA
Japan Food Research Laboratories, Tama Laboratory
Tetsuya HORIKITA
Laboratory of Animal Hygiene, Department of Veterinary Medicine, Faculty of Agriculture, Tokyo University of Agriculture and Technology
Tomoko HORISAKA
Laboratory of Animal Hygiene, Department of Veterinary Medicine, Faculty of Agriculture, Tokyo University of Agriculture and Technology
Takahide TANIGUCHI
Laboratory of Veterinary Microbiology, Department of Veterinary Medicine, Faculty of Agriculture, Tokyo University of Agriculture and Technology
Yukio KATO
Department of Veterinary Public Health II, School of Veterinary Medicine, Azabu University
Hideki HAYASHIDANI
Laboratory of Animal Hygiene, Department of Veterinary Medicine, Faculty of Agriculture, Tokyo University of Agriculture and Technology

Keywords: Erysipelothrix, riboprinter, ribotyping

JOURNAL FREE ACCESS

2004 Volume 66 Issue 6 Pages 729-733

DOI https://doi.org/10.1292/jvms.66.729

Details

Abstract

Automated ribotyping classified 70 Erysipelothrix species strains, previously classified into 14 RAPD patterns and into 63 PFGE patterns, into 27 ribogroups. Twenty-three strains of the 70 analyzed and classified into 13 ribogroups were previously classified into six ribotypes by the traditional ribotyping method. Moreover, automated ribotyping differentiated seven strains that were not differentiated by PFGE. Therefore, automated ribotyping was more sensitive than RAPD and traditional ribotyping, and it might be a useful method for a rapid screening in epidemiological study of strains of this genus, and more accurate results can be obtained when this method is used together with PFGE.

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