Enzootic bovine leukosis caused by bovine leukemia virus classified as Group          C based on viral whole genome sequencing in a 23-month-old Holstein-Friesian          heifer

Yusuke DAIJI; Masaki MAEZAWA; Kento ISHIKAWA; James K CHAMBERS; Kazuyuki UCHIDA; Hisashi INOKUMA

doi:10.1292/jvms.23-0354

Internal Medicine

Enzootic bovine leukosis caused by bovine leukemia virus classified as Group C based on viral whole genome sequencing in a 23-month-old Holstein-Friesian heifer

Yusuke DAIJI, Masaki MAEZAWA, Kento ISHIKAWA, James K CHAMBERS, Kazuyuki UCHIDA, Hisashi INOKUMA

Author information

Yusuke DAIJI
Central Veterinary Clinical Center, Agricultural Mutual Aid Association, Chiba, Japan
Masaki MAEZAWA
Laboratory of OSG Veterinary Science for Global Disease Management, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo, Japan
Kento ISHIKAWA
Laboratory of Veterinary Pathology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo, Japan
James K CHAMBERS
Laboratory of Veterinary Pathology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo, Japan
Kazuyuki UCHIDA
Laboratory of Veterinary Pathology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo, Japan
Hisashi INOKUMA
Laboratory of OSG Veterinary Science for Global Disease Management, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo, Japan
Veterinary Medical Center, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo, Japan

Keywords: bovine leukemia virus Group C strain, bovine leukemia virus, early onset, enzootic bovine leukosis, heifer

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Supplementary material

2023 Volume 85 Issue 12 Pages 1291-1295

DOI https://doi.org/10.1292/jvms.23-0354

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Abstract

A 23-month-old Holstein-Friesian heifer presented with inactivity and diarrhea. On physical examination, no enlargement of superficial lymph nodes was observed. Hematological examination revealed lymphocytosis. The bovine leukemia virus (BLV) proviral load was 2,122 copies/10 ng DNA, and BLV was classified as Group C based on whole genome phylogenetic analysis. Monoclonal proliferation of B-cells and monoclonal integration of the BLV provirus in the bovine genome were detected by a clonality test of B-cells and inverse PCR, respectively. Although lymph nodes were not swollen at necropsy, histopathological examination revealed neoplastic lymphocyte proliferation in lymph nodes, which were immune positive for CD5 and CD20, and negative for CD3. The heifer was diagnosed with EBL caused by BLV classified as Group C.

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