Comparison of antigenicity between recently isolated bovine rotaviruses A and vaccine strains by cross-neutralizing antibody tests and their significance for immunization

Md. Amirul HASAN; Md. Humayun KABIR; Yu MIYAOKA; Makiko YAMAGUCHI; Dany SHOHAM; Kazuaki TAKEHARA

doi:10.1292/jvms.22-0510

Virology

Comparison of antigenicity between recently isolated bovine rotaviruses A and vaccine strains by cross-neutralizing antibody tests and their significance for immunization

Md. Amirul HASAN, Md. Humayun KABIR, Yu MIYAOKA, Makiko YAMAGUCHI, Dany SHOHAM, Kazuaki TAKEHARA

Author information

Md. Amirul HASAN
Laboratory of Animal Health, Cooperative Division of Veterinary Sciences, Graduate School of Agriculture, Tokyo University of Agriculture and Technology, Tokyo, Japan
Md. Humayun KABIR
Laboratory of Animal Health, Cooperative Division of Veterinary Sciences, Graduate School of Agriculture, Tokyo University of Agriculture and Technology, Tokyo, Japan
Yu MIYAOKA
Laboratory of Animal Health, Cooperative Division of Veterinary Sciences, Graduate School of Agriculture, Tokyo University of Agriculture and Technology, Tokyo, Japan
Makiko YAMAGUCHI
Laboratory of Animal Health, Department of Veterinary Medicine, Faculty of Agriculture, Tokyo University of Agriculture and Technology, Tokyo, Japan
Dany SHOHAM
Bar-Ilan University, Begin-Sadat Center for Strategic Studies, Ramat Gan, Israel
Kazuaki TAKEHARA
Laboratory of Animal Health, Cooperative Division of Veterinary Sciences, Graduate School of Agriculture, Tokyo University of Agriculture and Technology, Tokyo, Japan
Laboratory of Animal Health, Department of Veterinary Medicine, Faculty of Agriculture, Tokyo University of Agriculture and Technology, Tokyo, Japan

Keywords: bovine rotavirus A, serotype, vaccine, virus isolation, virus neutralization test

JOURNAL OPEN ACCESS

2023 Volume 85 Issue 3 Pages 318-325

DOI https://doi.org/10.1292/jvms.22-0510

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Abstract

Three bovine rotaviruses A (RVAs) isolated from a cattle farm in Japan were serotyped by serum neutralization assay, as compared with the RVA strains contained in a vaccine used on the same farm. Antisera were prepared against the three isolates and the vaccine strains of bovine RVA. The results of cross-neutralization tests revealed that the RVA isolates from this farm differed somewhat in serotype. Collected plasma from calves for 6 weeks after colostrum ingestion showed that maternal antibodies acquired against all isolates gradually decreased, but antibodies toward one isolate increased by 6 weeks after the mentioned decreasing. These results suggest that rotavirus vaccines administered to cows should include all serotypes commonly found in calves with diarrhea.

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