Comparative evaluation of two ELISA kits for detecting antibodies to a          nonstructural protein of foot-and-mouth disease virus using serum samples collected from          naturally and experimentally infected cows

Katsuhiko FUKAI; Tatsuya NISHI; Nobuaki SHIMADA; Kazuki MORIOKA; Manabu YAMADA; Kazuo YOSHIDA; Makoto YAMAKAWA

doi:10.1292/jvms.17-0662

Virology

Comparative evaluation of two ELISA kits for detecting antibodies to a nonstructural protein of foot-and-mouth disease virus using serum samples collected from naturally and experimentally infected cows

Katsuhiko FUKAI, Tatsuya NISHI, Nobuaki SHIMADA, Kazuki MORIOKA, Manabu YAMADA, Kazuo YOSHIDA, Makoto YAMAKAWA

Author information

Katsuhiko FUKAI
Exotic Disease Research Station, National Institute of Animal Health, National Agriculture and Food Research Organization, 6-20-1 Josui-honcho, Kodaira, Tokyo 187-0022, Japan
Tatsuya NISHI
Exotic Disease Research Station, National Institute of Animal Health, National Agriculture and Food Research Organization, 6-20-1 Josui-honcho, Kodaira, Tokyo 187-0022, Japan
Nobuaki SHIMADA
Exotic Disease Research Station, National Institute of Animal Health, National Agriculture and Food Research Organization, 6-20-1 Josui-honcho, Kodaira, Tokyo 187-0022, Japan
Kazuki MORIOKA
Exotic Disease Research Station, National Institute of Animal Health, National Agriculture and Food Research Organization, 6-20-1 Josui-honcho, Kodaira, Tokyo 187-0022, Japan
Manabu YAMADA
Exotic Disease Research Station, National Institute of Animal Health, National Agriculture and Food Research Organization, 6-20-1 Josui-honcho, Kodaira, Tokyo 187-0022, Japan
Kazuo YOSHIDA
Exotic Disease Research Station, National Institute of Animal Health, National Agriculture and Food Research Organization, 6-20-1 Josui-honcho, Kodaira, Tokyo 187-0022, Japan
Makoto YAMAKAWA
Exotic Disease Research Station, National Institute of Animal Health, National Agriculture and Food Research Organization, 6-20-1 Josui-honcho, Kodaira, Tokyo 187-0022, Japan

Keywords: cattle, ELISA, Foot-and-mouth disease virus, nonstructural protein

JOURNAL FREE ACCESS

2018 Volume 80 Issue 10 Pages 1624-1630

DOI https://doi.org/10.1292/jvms.17-0662

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Abstract

When foot-and-mouth disease (FMD) occurs and a “vaccination-to-live” policy is adopted in a country, the country must perform serological surveillance of a nonstructural protein (NSP) of FMD virus. The NCPanaftosa kit is the only kit for detecting antibodies to NSPs that is officially recognized as the reference regent by the World Organization for Animal Health; however, it is only used in South American countries. In this study, the specificity and sensitivity of the NCPanaftosa kit were compared with those of the PrioCHECK kit sold by an international company. Results in this study suggest that the PrioCHECK kit performs similarly to the NCPanaftosa kit in detecting antibodies to the NSP in the cattle population.

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