Prior exposure to Bordetella species as an exclusion criterion in the baboon model of pertussis

Annalee W. NGUYEN; Ellen K. WAGNER; Luciano POSADA; Xinlei LIU; Sheila CONNELLY; James F. PAPIN; Roman F. WOLF; Michael KALEKO; Jennifer A. MAYNARD

doi:10.1292/jvms.16-0427

Laboratory Animal Science

Prior exposure to Bordetella species as an exclusion criterion in the baboon model of pertussis

Annalee W. NGUYEN, Ellen K. WAGNER, Luciano POSADA, Xinlei LIU, Sheila CONNELLY, James F. PAPIN, Roman F. WOLF, Michael KALEKO, Jennifer A. MAYNARD

Author information

Annalee W. NGUYEN
Department of Chemical Engineering, The University of Texas at Austin, Austin, TX 78712, U.S.A.
Ellen K. WAGNER
Department of Chemical Engineering, The University of Texas at Austin, Austin, TX 78712, U.S.A.
Luciano POSADA
Department of Chemical Engineering, The University of Texas at Austin, Austin, TX 78712, U.S.A.
Xinlei LIU
Department of Chemical Engineering, The University of Texas at Austin, Austin, TX 78712, U.S.A.
Sheila CONNELLY
Synthetic Biologics, 9605 Medical Center Drive, Suite 270, Rockville, MD 20850, U.S.A.
James F. PAPIN
Department of Comparative Medicine, University of Oklahoma Health Sciences Center, Oklahoma City, OK 73104, U.S.A.
Roman F. WOLF
Department of Comparative Medicine, University of Oklahoma Health Sciences Center, Oklahoma City, OK 73104, U.S.A.
Michael KALEKO
Synthetic Biologics, 9605 Medical Center Drive, Suite 270, Rockville, MD 20850, U.S.A.
Jennifer A. MAYNARD
Department of Chemical Engineering, The University of Texas at Austin, Austin, TX 78712, U.S.A.

Keywords: baboon, Bordetella bronchiseptica, Bordetella pertussis, filamentous hemagglutinin, whooping cough

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Supplementary material

2017 Volume 79 Issue 1 Pages 60-64

DOI https://doi.org/10.1292/jvms.16-0427

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Abstract

The baboon model of Bordetella pertussis infection is the newest and most clinically accurate model of the human disease to date. However, among the 15 experimentally infected baboons in this study, a subset of baboons did not exhibit the expected high bacterial colonization levels or increase in white blood cell count. Moreover, cultures of nasopharyngeal wash samples from several baboons suggested B. bronchiseptica coinfection. Analysis of serum antibodies recognizing filamentous hemagglutinin, pertussis toxin and B. pertussis lipo-oligosaccharide indicated that several baboons had likely been previously exposed to Bordetella species and that prior exposure correlated with partial protection from B. pertussis infection. Notably, all animals with a baseline Fha titer of 5 IU/ml or below exhibited symptoms typical of the model, suggesting this value can be used as inclusion criteria for animals prior to study enrollment. While B. pertussis infection is endemic to human populations and B. bronchiseptica is common in wild small mammals, this study illustrates that baboons can readily harbor both organisms. Awareness of Bordetella species that share antigens capable of generating protective immune responses and tracking of prior exposure to those species is required for successful use of the baboon model of pertussis.

Corresponding author

Funder information

1.Fund name: National Institutes of Health

2.Fund name: Synthetic Biologics

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