Infectivity of Cryptosporidium andersoni and Cryptosporidium muris to Normal and Immunosuppressive Cynomolgus Monkeys

Koichi MASUNO; Yasuhiro FUKUDA; Masahito KUBO; Ryo IKARASHI; Takeshi KURAISHI; Shosaku HATTORI; Junpei KIMURA; Chieko KAI; Tokuma YANAI; Yutaka NAKAI

doi:10.1292/jvms.13-0350

Parasitology

Infectivity of Cryptosporidium andersoni and Cryptosporidium muris to Normal and Immunosuppressive Cynomolgus Monkeys

Koichi MASUNO, Yasuhiro FUKUDA, Masahito KUBO, Ryo IKARASHI, Takeshi KURAISHI, Shosaku HATTORI, Junpei KIMURA, Chieko KAI, Tokuma YANAI, Yutaka NAKAI

Author information

Koichi MASUNO
Drug Developmental Research Laboratories, Shionogi & Co., Ltd., 3–1–1, Futaba-Cho, Toyonaka, Osaka 561–0825, Japan
Veterinary Medicine, Gifu University, 1–1 Yanagido, Gifu, Gifu 501–1194, Japan
Yasuhiro FUKUDA
Laboratory of Sustainable Environmental Biology, Graduate School of Agricultural Science, Tohoku University, Naruko-Onsen, Osaki, Miyagi 989–6711, Japan
Masahito KUBO
Veterinary Medicine, Gifu University, 1–1 Yanagido, Gifu, Gifu 501–1194, Japan
Laboratory of Veterinary Pathology, Yamaguchi University, 1677–1 Yoshida, Yamaguchi-City, Yamaguchi 753–8511, Japan
Ryo IKARASHI
Laboratory of Sustainable Environmental Biology, Graduate School of Agricultural Science, Tohoku University, Naruko-Onsen, Osaki, Miyagi 989–6711, Japan
Takeshi KURAISHI
The Institute of Medical Science, The University of Tokyo, 4–6–1 Shirogane-dai, Minato-ku, Tokyo 108–8639, Japan
Shosaku HATTORI
The Institute of Medical Science, The University of Tokyo, 4–6–1 Shirogane-dai, Minato-ku, Tokyo 108–8639, Japan
Junpei KIMURA
Korea Secretary General of Asian Society of Zoo and Wildlife Medicine, College of Veterinary Medicine, Seoul National University, Seoul 151–742, Korea
Chieko KAI
The Institute of Medical Science, The University of Tokyo, 4–6–1 Shirogane-dai, Minato-ku, Tokyo 108–8639, Japan
Tokuma YANAI
Veterinary Medicine, Gifu University, 1–1 Yanagido, Gifu, Gifu 501–1194, Japan
Yutaka NAKAI
Laboratory of Sustainable Environmental Biology, Graduate School of Agricultural Science, Tohoku University, Naruko-Onsen, Osaki, Miyagi 989–6711, Japan

Keywords: Cryptosporidium, experimental animals, monkey, parasitology, pathology

JOURNAL FREE ACCESS

2014 Volume 76 Issue 2 Pages 169-172

DOI https://doi.org/10.1292/jvms.13-0350

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Abstract

Cryptosporidium andersoni and Cryptosporidium muris infections have been found in the mice and/or cattle. The oocysts of C. andersoni and C. muris have been sporadically detected in human feces, but the infectious capacity and features have been unknown, because of the scarcity of reports involving human infections. To assess the infectivity and the clinical and pathological features of C. andersoni and C. muris in primates, an experimental infectious study was conducted using cynomolgus monkeys. The monkeys were orally inoculated with oocysts of two different C. andersoni Kawatabi types and C. muris RN-66 under normal and immunosuppressive conditions. The feces of the monkeys were monitored for about 40 days after the administration of oocysts using the flotation method, but no shedding oocysts were observed under either both normal or immunosuppressive conditions. Gross and histopathological examinations were performed on the immunosuppressive monkeys, but these revealed no evidence of Cryptosporidium infections, even though the monkeys were subjected to immunosuppressive conditions. It is hypothesized that C. andersoni and C. muris pose little danger of infection in primates even under immunosuppressive conditions.

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