Rapid diagnosis of equine influenza by highly sensitive silver amplification          immunochromatography system

Takashi YAMANAKA; Manabu NEMOTO; Hiroshi BANNAI; Koji TSUJIMURA; Takashi KONDO; Tomio MATSUMURA; Tao Qi Huang FU; Charlene Judith FERNANDEZ; Sarah GILDEA; Ann CULLINANE

doi:10.1292/jvms.17-0105

Virology

Rapid diagnosis of equine influenza by highly sensitive silver amplification immunochromatography system

Takashi YAMANAKA, Manabu NEMOTO, Hiroshi BANNAI, Koji TSUJIMURA, Takashi KONDO, Tomio MATSUMURA, Tao Qi Huang FU, Charlene Judith FERNANDEZ, Sarah GILDEA, Ann CULLINANE

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Keywords: diagnosis, equine influenza, rapid antigen detection, silver amplification

JOURNAL FREE ACCESS

2017 Volume 79 Issue 6 Pages 1061-1063

DOI https://doi.org/10.1292/jvms.17-0105

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Abstract

Equine inﬂuenza (EI) is a respiratory disease caused by equine influenza A virus (EIV, H3N8) infection. Rapid diagnosis is essential to limit the disease spread. We previously reported that some rapid antigen detection (RAD) tests are ﬁt for diagnosing EI although their sensitivity is not optimal. Here, we evaluated the performance of the newly developed RAD test using silver amplification immunochromatography (Quick Chaser Auto Flu A, B: QCA) to diagnose EI. The detection limits of QCA for EIVs were five-fold lower than the conventional RAD tests. The duration of virus antigen detection in the infected horses was longer than the conventional RAD tests. We conclude that QCA could be a valuable diagnostic method for EI.

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