Abstract—Endophytes are fungi associated with plants without causing symptoms. They are quite diverse in nature and have enormous potential for production of important secondary metabolites for the pharmaceutical industry. Thus the aim of this work was to isolate an endophytic fungal strain possessing antimicrobial activity against the selected human pathogens. In this study we report for the first time microwave assisted extraction of secondary metabolites from an endophytic fungal strain NFX06 isolated from leaf of Nothapodytes foetida of Agumbe forest, Karnataka. The fungal strain was identified as Fusarium oxysporum NFX06 based on its macroscopical and microscopical characteristics. Further confirmation of the species was done by Internal Transcribed Spacer (ITS) sequencing and the nucleotide sequence was submitted to the GenBank with an accession number KC914432. The highest activity against all the four pathogenic strains [Staphylococcus aureus (ATCC 25923), Escherichia coli (ATCC 25922) Pseudomonas aeruginosa (ATCC 27853) and Candida albicans (ATCC 69548)] were exhibited by ethyl acetate extract with a Minimum Bactericidal Concentration (MBC) of about 30μg/mL against Staphylococcus aureus and a Minimum Fungicidal Concentration (MFC) of about 50μg/mL against Candida albicans.

Index Terms—endophytic fungi, sequencing, microwave extraction, minimum inhibitory concentration, Nothapodytes foetida

Cite: Sogra Fathima Musavi and Raj Mohan Balakrishnan, "A Study on the Antimicrobial Potentials of an Endophytic Fungus Fusarium oxysporum NFX 06," Journal of Medical and Bioengineering, Vol. 3, No. 3, pp. 162-166, September 2014. Doi: 10.12720/jomb.3.3.162-166