26 August 2019 : Clinical Research
Association of Platelet Membrane Fatty Acid Composition with Markers of Oxidative Stress in Healthy Men
Inga Bikulčienė1ABCDEF*, Orinta Golubevaitė1BCDF, Vytautas Žėkas1AB, Mantas Radzevičius1B, Dovilė Karčiauskaitė1ABCDEFG, Rėda Matuzevičienė1ABCD, Vaiva Hendrixson1DE, Asta Mažeikienė1B, Neringa Burokienė2B, Arvydas Kaminskas1ACD, Zita Aušrelė Kučinskienė1ADGDOI: 10.12659/MSM.915111
Med Sci Monit 2019; 25:6405-6416
Abstract
BACKGROUND: Platelet membranes are extremely susceptible to peroxidation, forming a variety of lipid peroxides, including malondialdehyde (MDA), which has been implicated in the etiology of cardiovascular diseases. Moreover, platelet-leukocyte aggregates (PLAs) are known to contribute to advanced endothelial injury and atherogenesis.
MATERIAL AND METHODS: Fatty acid (FA) methyl esters of the platelet membranes of 79 apparently healthy men without any acute clinical condition at the time of the study were identified by GC/MS. MDA was measured by HPLC in blood serum, and PLAs were analyzed by whole-blood flow cytometry. Individuals were divided into quartiles according to MDA concentration and percentage of PLAs formation. The composition of platelet membrane FAs was compared to MDA concentration and the percentage of PLAs formation in apparently healthy individuals.
RESULTS: In quartiles (Q) with higher MDA concentration, percentage of C 16: 1ω7 (Q₁ vs. Q₃, p=0.021), C 20: 1ω9 (Q₂ vs. Q₄, p=0.028) and C 20: 5ω3 (Q₂ vs. Q₄, p=0.046) was lower. However, C 22: 5ω3 (Q₁ vs. Q₄, p=0.038) and total ω3 (Q₁ vs. Q₂, p=0.024) were higher.
CONCLUSIONS: MDA and the formation of platelet–monocyte aggregates stimulate the incorporation of monounsaturated fatty acids and polyunsaturated fatty acids in platelet phospholipid membranes, which may be a hallmark for a changed level of biologically active compounds required for the activation of future platelets.
Keywords: Malondialdehyde, Platelet Activation, Blood Platelets, Fatty Acids, Fatty Acids, Monounsaturated, Fatty Acids, Unsaturated, Gas Chromatography-Mass Spectrometry, healthy volunteers, Lipid Peroxidation, Phospholipids, Platelet Aggregation
Editorial
01 May 2024 : Editorial
Editorial: First Regulatory Approval for Adoptive Cell Therapy with Autologous Tumor-Infiltrating Lymphocytes (TILs) – Lifileucel (Amtagvi)DOI: 10.12659/MSM.944927
Med Sci Monit 2024; 30:e944927
In Press
Clinical Research
The Influence of Gestational Diabetes Mellitus on Maternal and Neonatal Outcomes: A Retrospective Study in ...Med Sci Monit In Press; DOI: 10.12659/MSM.943644
Clinical Research
Exploring the Educational Social and Physical Activities Among Health Care Undergraduates – a Cross-Section...Med Sci Monit In Press; DOI: 10.12659/MSM.943399
Clinical Research
Percutaneous Coronary Intervention as a Non-Surgical Treatment for Ischemic Mitral Regurgitation in Patient...Med Sci Monit In Press; DOI: 10.12659/MSM.943122
Clinical Research
Maxillary Canine Impaction: Assessing the Influence of Maxillary Anatomy Using Cone Beam Computed TomographyMed Sci Monit In Press; DOI: 10.12659/MSM.944306
Most Viewed Current Articles
17 Jan 2024 : Review article
Vaccination Guidelines for Pregnant Women: Addressing COVID-19 and the Omicron VariantDOI :10.12659/MSM.942799
Med Sci Monit 2024; 30:e942799
14 Dec 2022 : Clinical Research
Prevalence and Variability of Allergen-Specific Immunoglobulin E in Patients with Elevated Tryptase LevelsDOI :10.12659/MSM.937990
Med Sci Monit 2022; 28:e937990
16 May 2023 : Clinical Research
Electrophysiological Testing for an Auditory Processing Disorder and Reading Performance in 54 School Stude...DOI :10.12659/MSM.940387
Med Sci Monit 2023; 29:e940387
01 Jan 2022 : Editorial
Editorial: Current Status of Oral Antiviral Drug Treatments for SARS-CoV-2 Infection in Non-Hospitalized Pa...DOI :10.12659/MSM.935952
Med Sci Monit 2022; 28:e935952