Myo2p, a class V myosin in budding yeast, associates with a large ribonucleic acid–protein complex that contains mRNAs and subunits of the RNA-processing body

Wakam Chang; Rania F. Zaarour; Samara Reck-Peterson; John Rinn; Robert H. Singer; Michael Snyder; Peter Novick; Mark S. Mooseker

doi:10.1261/rna.665008

Myo2p, a class V myosin in budding yeast, associates with a large ribonucleic acid–protein complex that contains mRNAs and subunits of the RNA-processing body

¹Department of Molecular, Cellular and Developmental Biology, Yale University, New Haven, Connecticut 06520, USA
²Department of Cell Biology, Yale University School of Medicine, New Haven, Connecticut 06511, USA
³Anatomy and Structural Biology, Albert Einstein College of Medicine, Bronx, New York 10461, USA
⁴Department of Pathology, Yale University School of Medicine, New Haven, Connecticut 06511, USA

↵5 These authors contributed equally to this work.

Abstract

Myo2p is an essential class V myosin in budding yeast with several identified functions in organelle trafficking and spindle orientation. The present study demonstrates that Myo2p is a component of a large RNA-containing complex (Myo2p–RNP) that is distinct from polysomes based on sedimentation analysis and lack of ribosomal subunits in the Myo2p–RNP. Microarray analysis of RNAs that coimmunoprecipitate with Myo2p revealed the presence of a large number of mRNAs in this complex. The Myo2p–RNA complex is in part composed of the RNA processing body (P-body) based on coprecipitation with P-body protein subunits and partial colocalization of Myo2p with P-bodies. P-body disassembly is delayed in the motor mutant, myo2-66, indicating that Myo2p may facilitate the release of mRNAs from the P-body.

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Footnotes

↵6 Present addresses: UMR 144 CNRS-Institut Curie 26 rue d'Ulm, 75248 PARIS cedex 05, France;
↵7 Department of Cell Biology, Harvard Medical School, 250 Longwood Avenue, Boston, MA 02115, USA;
↵8 Program in Epithelial Biology, Department of Dermatology, Stanford University School of Medicine, Stanford, CA 94305, USA.
Reprint requests to: Mark S. Mooseker, Department of Molecular, Cellular and Developmental Biology, 352 KBT, Yale University, New Haven, CT 06520, USA; e-mail: mark.mooseker{at}yale.edu; fax: (203) 432-6161.
Article published online ahead of print. Article and publication date are at http://www.rnajournal.org/cgi/doi/10.1261/rna.665008.
- Received May 30, 2007.
- Accepted November 21, 2007.