α-Phenyl-N-tert-butyl Nitrone Has Scavenging Activity Against Singlet Oxygen (1O2) and Attenuates 1O2-Induced Neuronal Cell Death

Anna Kawai; Yoko Nishinaka; Toshiyuki Arai; Kiichi Hirota; Hiroko Mori; Nobuyuki Endo; Takashi Miyoshi; Kouhei Yamashita; Masataka Sasada

doi:10.1254/jphs.08233SC

Short Communication

α-Phenyl-N-tert-butyl Nitrone Has Scavenging Activity Against Singlet Oxygen (¹O₂) and Attenuates ¹O₂-Induced Neuronal Cell Death

Anna Kawai, Yoko Nishinaka, Toshiyuki Arai, Kiichi Hirota, Hiroko Mori, Nobuyuki Endo, Takashi Miyoshi, Kouhei Yamashita, Masataka Sasada

Author information

Anna Kawai
School of Health Science, Faculty of Medicine, Kyoto University, Japan
Yoko Nishinaka
School of Health Science, Faculty of Medicine, Kyoto University, Japan
Toshiyuki Arai
Department of Anesthesia, Kyoto University Hospital, Japan
Kiichi Hirota
Department of Anesthesia, Kyoto University Hospital, Japan
Hiroko Mori
Department of Anesthesia, Higashiyama Takeda Hospital, Japan
Nobuyuki Endo
Wakasa Wan Energy Research Center, Japan
Takashi Miyoshi
Department of Hematology and Oncology, Kyoto University Hospital, Japan
Kouhei Yamashita
Department of Hematology and Oncology, Kyoto University Hospital, Japan
Masataka Sasada
School of Health Science, Faculty of Medicine, Kyoto University, Japan

Keywords: α-phenyl-N-tert-butyl nitrone, singlet oxygen, neuronal cell death

JOURNAL FREE ACCESS

2008 Volume 108 Issue 4 Pages 545-549

DOI https://doi.org/10.1254/jphs.08233SC

View "Advance Publication" version (December 10, 2008).

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Abstract

Scavenging activity of α-phenyl-N-tert-butyl nitrone (PBN) against singlet oxygen (¹O₂) and its effects on ¹O₂-induced neuronal cell death were examined. PBN at 1 – 4 mM dose-dependently suppressed ¹O₂ released from activated human neutrophils. PBN did not react with hydrogen peroxide or hypochlorite and did not affect myeloperoxidase activity, which are involved in the ¹O₂ formation in neutrophils. PBN also suppressed chemically generated ¹O₂ in a cell-free system. These findings collectively indicated that PBN certainly has scavenging activity against ¹O₂. Furthermore, PBN attenuated ¹O₂-induced neuronal cell death. The well-known neuroprotective effects of PBN might be attributed to its ¹O₂-scavenging activity.

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