2011 Volume 59 Issue 2 Pages 202-207
We have engineered a novel, non-viral, multifunctional gene vector (STR-CH2R4H2C) that contained stearoyl (STR) and a block peptide consisting of Cys (C), His (H), and Arg (R). STR-CH2R4H2C can form a stable nano-complex with plasmid DNA (pDNA) based on electronic interactions and disulfide cross linkages. In this study, we evaluated the efficacy of STR-CH2R4H2C as a gene vector. We first determined the optimal weight ratio for STR-CH2R4H2C/pDNA complexes. The complexes with a weight ratio of 50 showed the highest transfection efficacy. We also examined the transfection efficacy of STR-CH2R4H2C/pDNA complexes with or without serum and compared STR-CH2R4H2C/pDNA transfection efficacy with that of Lipofectamine. Even in the presence of serum, STR-CH2R4H2C showed higher transfection efficacy than did Lipofectamine. In addition, we determined the mechanism of transfection of the STR-CH2R4H2C/pDNA complexes using various cellular uptake inhibitors and evaluated its endosomal escape ability using chloroquine. Macropinocytosis was main cellular uptake pathway of STR-CH2R4H2C/pDNA complexes. Our results suggested that STR-CH2R4H2C is a promising gene delivery system.
