Three new butanolides, 2-(1-methoxy-11-dodecenyl)-penta-2, 4-dien-4-olide (1), (2Z, 3S, 4S)-2-(11-dodecenylidene)-3-hydroxy-4-methylbutanolide (2) and (2E, 3R, 4R)-2-(11-dodecenylidene)-3-hydroxy-4-methoxy-4-methyl-butanolide (3), were isolated from the stems of Lindera obtusiloba BLUME. Their chemical structures were assigned by spectroscopic evidence. They exhibited cytotoxicity against cultured human tumor cell lines with their ED₅₀ values ranging from 3.19 to 14.63 μg/ml.