Studies on Peptides. CLVII. : Synthesis of a Frog-Skin Peptide, Sauvagine

MOTOYOSHI NOMIZU; KENICHI AKAJI; JUNICHI FUKATA; HIROO IMURA; ATSUKO INOUE; YOSHIHIRO NAKATA; TOMIO SEGAWA; NOBUTAKA FUJII; HARUAKI YAJIMA

doi:10.1248/cpb.36.122

MOTOYOSHI NOMIZU, KENICHI AKAJI, JUNICHI FUKATA, HIROO IMURA, ATSUKO INOUE, YOSHIHIRO NAKATA, TOMIO SEGAWA, NOBUTAKA FUJII, HARUAKI YAJIMA

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MOTOYOSHI NOMIZU
KENICHI AKAJI
JUNICHI FUKATA
HIROO IMURA
ATSUKO INOUE
YOSHIHIRO NAKATA
TOMIO SEGAWA
NOBUTAKA FUJII
HARUAKI YAJIMA
Author's Organization：
Faculty of Pharmaceutical Sciences, Kyoto University
Faculty of Pharmaceutical Sciences, Kyoto University
the 2nd Internal Medicine, Faculty of Medicine, Kyoto University
the 2nd Internal Medicine, Faculty of Medicine, Kyoto University
Faculty of Pharmaceutical Sciences, School of Medicine, Hiroshima University
Faculty of Pharmaceutical Sciences, School of Medicine, Hiroshima University
Faculty of Pharmaceutical Sciences, School of Medicine, Hiroshima University
Faculty of Pharmaceutical Sciences, Kyoto University
Faculty of Pharmaceutical Sciences, Kyoto University

Keywords: hypotensive action

JOURNAL FREE ACCESS

1988 Volume 36 Issue 1 Pages 122-133

DOI https://doi.org/10.1248/cpb.36.122

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Abstract

A tetracontapeptide corresponding to the entire amino acid sequence of a frog skin peptide, sauvagine, was synthesized by assembling eight peptide fragments of established purity followed by deprotection with 1 M trimethylsilyl trifluoromethanesulfonate-thioanisole in trifluoroacetic acid. The synthetic peptide stimulated secretion of immunoreactive corticotropin from rat pituitary cells and its potency was estimated to be equivalent to that of synthetic ovine corticotropin releasing factor. Synthetic sauvagine displayed long-lasting hypotensive action in rats.

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