Studies on Peptides. CXLVI. : Synthesis of Gln15-Motilin and Examination of Its Immunological Properites

SUZUMITSU KUNO; WEI LI; NOBUTAKA FUJII; HIDEKI ADACHI; KIYOSHI BESSYO; TOMIO SEGAWA; YOSHIHIRO NAKATA; ATSUKO INOUE; HARUAKI YAJIMA

doi:10.1248/cpb.34.4811

Studies on Peptides. CXLVI. : Synthesis of Gln¹⁵-Motilin and Examination of Its Immunological Properites

SUZUMITSU KUNO, WEI LI, NOBUTAKA FUJII, HIDEKI ADACHI, KIYOSHI BESSYO, TOMIO SEGAWA, YOSHIHIRO NAKATA, ATSUKO INOUE, HARUAKI YAJIMA

Author information

SUZUMITSU KUNO
WEI LI
NOBUTAKA FUJII
HIDEKI ADACHI
KIYOSHI BESSYO
TOMIO SEGAWA
YOSHIHIRO NAKATA
ATSUKO INOUE
HARUAKI YAJIMA
Author's Organization：
Faculty of Pharmaceutical Science, Kyoto University
Faculty of Pharmaceutical Science, Kyoto University
Faculty of Pharmaceutical Science, Kyoto University
University Hospital, Faculty of Medicine, Kyoto University
College of Liberal Arts, Kyoto University
Institute of Pharmaceutical Sciences, School of Medicine, Hiroshima University
Institute of Pharmaceutical Sciences, School of Medicine, Hiroshima University
Institute of Pharmaceutical Sciences, School of Medicine, Hiroshima University
Faculty of Pharmaceutical Science, Kyoto University

Keywords: motilin immunogenicity

JOURNAL FREE ACCESS

1986 Volume 34 Issue 11 Pages 4811-4816

DOI https://doi.org/10.1248/cpb.34.4811

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Abstract

Gln¹⁵-motilin was synthesized in a conventional manner by assembling seven peptide fragments followed by deprotection with 1M trifluoromethanesulfonic acid-thioanisole in trifluoroacetic acid. The usefulness of phenylthiotrimethylsilane for the reduction of Met(O) was confirmed. This motilin analog was as biologically active as synthetic motilin in terms of contraction of rabbit duodenal muscles, but in radioimmunoassay it was only about 25% as reactive with antiserum raised against synthetic porcine motilin.

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