Dicyclohexylamine (DCHA), a metabolite of sodium cyclamate (CHS-Na), was identified by gas liquid chromatography (GLC) and thin-layer chromatography (TLC) from the urine of rabbits and rats following repeated oral administration of CHS-Na. The urinary excretions (% of the dose) of DCHA in rabbits and rats receiving CHS-Na were 0.041×10^-3 and 0.143×10^-3%, respectively. Moreover, the metabolic fate of DCHA was investigated in rabbits and rats. The urinary and fecal excretions of unchanged DCHA were relatively small in both rabbits and rats receiving oral or intravenous administration of DCHA. In addition, DCHA was suggested to be primarily metabolized by oxidative reaction in the hepatic 10000×g supernatant. Thus, it may be due to further metabolism of the produced DCHA that only a small amount of DCHA is excreted in the urine after repeated oral administration of CHS-Na to rabbits and rats.