Application of High-performance Liquid Chromatography to the Isolation of Ginsenoside-Rf, -Rg2, and -Rh1 from a Crude Saponin Mixture of Ginseng

TETSURO NAGASAWA; JINHO CHOI; YOSHIE NISHINO; HIKOKICHI OURA

doi:10.1248/cpb.28.3701

Application of High-performance Liquid Chromatography to the Isolation of Ginsenoside-Rf, -Rg₂, and -Rh₁ from a Crude Saponin Mixture of Ginseng

TETSURO NAGASAWA, JINHO CHOI, YOSHIE NISHINO, HIKOKICHI OURA

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Keywords: isolation

JOURNAL FREE ACCESS

1980 Volume 28 Issue 12 Pages 3701-3707

DOI https://doi.org/10.1248/cpb.28.3701

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Abstract

The minor components of ginseng saponins, ginsenoside-Rf, -Rg₂, and -Rh₁, were isolated from the crude saponin fraction by a high-performance liquid chromatography (HPLC) procedure involving preparative HPLC on silica gel followed by semi-preparative HPLC on a column packing of Carbohydrate Analysis. This method was rapid and convenient.

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