1974 Volume 22 Issue 7 Pages 1571-1579
A fluorescent probe specific for sulfhydryl group was synthesized. Conformational change in HSA was observed by fluorescence spectral shift on binding. From the experimental evidences of urea denaturation and fluorometric pH titration, phenylbutazone or secobarbital binding was concluded to induce the conformational change of HSA from the native state to a more expanded form.