Saucerneol G, a New Lignan, from Saururus chinensis Inhibits Matrix Metalloproteinase-9 Induction via a Nuclear Factor κB and Mitogen Activated Protein Kinases in Lipopolysaccharide-Stimulated RAW264.7 Cells

Yue Lu; Tae Gyun Hong; Meihua Jin; Ju Hae Yang; Seok-Jong Suh; Dong Gen Piao; Hyun-Kwon Ko; Chang-Seob Seo; Young-Chae Chang; Cheorl-Ho Kim; Jong-Keun Son; Hyeun Wook Chang

doi:10.1248/bpb.33.1944

Regular Articles

Saucerneol G, a New Lignan, from Saururus chinensis Inhibits Matrix Metalloproteinase-9 Induction via a Nuclear Factor κB and Mitogen Activated Protein Kinases in Lipopolysaccharide-Stimulated RAW264.7 Cells

Yue Lu, Tae Gyun Hong, Meihua Jin, Ju Hae Yang, Seok-Jong Suh, Dong Gen Piao, Hyun-Kwon Ko, Chang-Seob Seo, Young-Chae Chang, Cheorl-Ho Kim, Jong-Keun Son, Hyeun Wook Chang

Author information

Yue Lu
College of Pharmacy, Yeungnam University
Tae Gyun Hong
College of Pharmacy, Yeungnam University
Meihua Jin
College of Pharmacy, Yeungnam University
Ju Hae Yang
College of Pharmacy, Yeungnam University
Seok-Jong Suh
College of Pharmacy, Yeungnam University
Dong Gen Piao
College of Pharmacy, Yeungnam University
Hyun-Kwon Ko
Department of Biological Science, Sungkyunkwan University
Chang-Seob Seo
Herbal Medicine EBM Research Center, Korea Institute of Oriental Medicine
Young-Chae Chang
Department of Pathology, Catholic University of Daegu School of Medicine
Cheorl-Ho Kim
Department of Biological Science, Sungkyunkwan University
Jong-Keun Son
College of Pharmacy, Yeungnam University
Hyeun Wook Chang
College of Pharmacy, Yeungnam University

Keywords: saucerneol G, Saururus chinensis, matrix metalloproteinase-9, nuclear factor-κB, mitogen-activated protein kinase, lipopolysaccharide

JOURNAL FREE ACCESS

2010 Volume 33 Issue 12 Pages 1944-1948

DOI https://doi.org/10.1248/bpb.33.1944

Details

Abstract

This study was conducted to demonstrate the inhibitory effect of saucerneol G (SG), a new lignan, isolated from the aerial part of Saururus chinensis (Saururaceae) on lipopolysaccharide (LPS)-stimulated matrix metalloproteinase-9 (MMP)-9 inductions in RAW 264.7 cells. Aimed at evaluating the mechanism of action by which SG inhibits the LPS-mediated induction of MMP-9, the effects of SG on nuclear factor-κB (NF-κB) DNA binding activity, NF-κB-dependent reporter gene activity, inhibitory factor-κB (IκB) phosphorylation, degradation and p65 nuclear translocation were assessed. SG profoundly suppressed the DNA binding activity and the reporter gene activity as well as translocation of NF-κB p65 subunit. Furthermore, SG also dose dependently inhibited LPS-stimulated activation of mitogen-activated protein kinases (MAPKs). These findings suggest that SG may inhibit LPS-stimulated MMP-9 induction by blocking NF-κB and MAPKs activation.

Corresponding author

Register with J-STAGE for free!