In Vitro Antiviral Activity of Marine Sponges Collected Off Brazilian Coast

Alexandre Cordeiro da Silva; Jadel Muller Kratz; Fabiane Maria Farias; Amélia Terezinha Henriques; Josivete dos Santos; Rosa Maria Leonel; Clea Lerner; Beatriz Mothes; Célia Regina Monte Barardi; Cláudia Maria Oliveira Simões

doi:10.1248/bpb.29.135

Regular Articles

In Vitro Antiviral Activity of Marine Sponges Collected Off Brazilian Coast

Alexandre Cordeiro da Silva, Jadel Muller Kratz, Fabiane Maria Farias, Amélia Terezinha Henriques, Josivete dos Santos, Rosa Maria Leonel, Clea Lerner, Beatriz Mothes, Célia Regina Monte Barardi, Cláudia Maria Oliveira Simões

Author information

Alexandre Cordeiro da Silva
Laboratório de Virologia Aplicada da UFSC, Departamento de Ciências Farmacêuticas, CCS, Universidade Federal de Santa Catarina (UFSC), Brazil
Jadel Muller Kratz
Laboratório de Virologia Aplicada da UFSC, Departamento de Ciências Farmacêuticas, CCS, Universidade Federal de Santa Catarina (UFSC), Brazil
Fabiane Maria Farias
Faculdade de Farmácia, Universidade Federal do Rio Grande do Sul (UFRGS), Brazil
Amélia Terezinha Henriques
Faculdade de Farmácia, Universidade Federal do Rio Grande do Sul (UFRGS), Brazil
Josivete dos Santos
Departamento de Oceanografia, Universidade Federal de Pernambuco (UFPE), Brazil
Rosa Maria Leonel
Departamento de Sistemática e Ecologia, Universidade Federal da Paraíba (UFPB), Brazil
Clea Lerner
Fundação Zoobotânica, Museu de Ciências Naturais
Beatriz Mothes
Fundação Zoobotânica, Museu de Ciências Naturais
Célia Regina Monte Barardi
Laboratório de Virologia Aplicada da UFSC, Departamento de Microbiologia e Parasitologia, CCB, Universidade Federal de Santa Catarina (UFSC), Brazil
Cláudia Maria Oliveira Simões
Laboratório de Virologia Aplicada da UFSC, Departamento de Ciências Farmacêuticas, CCS, Universidade Federal de Santa Catarina (UFSC), Brazil

Keywords: antiviral activity, Brazilian marine sponge, MTT assay, HSV-1, adenovirus 5, rotavirus SA11

JOURNAL FREE ACCESS

2006 Volume 29 Issue 1 Pages 135-140

DOI https://doi.org/10.1248/bpb.29.135

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Abstract

This paper describes the in vitro antiviral evaluation of 27 different marine sponges (Porifera) collected off Brazilian coastline in the search for novel drug leads. With these sponges aqueous and organic extracts were prepared and tested for anti-herpetic (HSV-1, KOS strain), anti-adenovirus (human AdV serotype 5) and anti-rotavirus (simian RV SA11) activities. The evaluation of the cytotoxicity and potential antiviral activity of these extracts were performed by using MTT assay. Results were expressed as 50% cytotoxicity (CC₅₀) and 50% effective (EC₅₀) concentrations, respectively, in order to calculate the selectivity indices (SI=CC₅₀/EC₅₀) of each extract. From the 40 sponge extracts tested, 17 extracts showed antiviral action in different degrees. The results concerning the antiviral activity were obtained by using three different strategies: (1) simultaneous assay, when sponge extracts were added to the cells at the same time of the viruses; (2) pre treatment assay, when sponge extracts were added to the cells 15 h prior to the viruses infection; and (3) post treatment assay, when the viruses were added to the cells and remained during 2 h prior to the addition of sponge extracts. The antiviral assays with HSV-1/KOS and AdV-5 showed more promising results when the pre treatment test was employed. In relation to the RV-SA11 virus, only the simultaneous assay showed antiviral activity. The extracts presenting the most promising results were the aqueous extracts of Cliona sp., Agelas sp.2, Tethya sp., Axinella aff corrugata, Polymastia janeirensis and Protosuberites sp., and these extracts deserve special attention in further studies.

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