Effect of Cationic Liposomes in an in Vitro Transcription and Translation System

Rieko Tachibana; Hideyoshi Harashima; Tatsuhiro Ishida; Yasuo Shinohara; Mami Hino; Hiroshi Terada; Yoshinobu Baba; Hiroshi Kiwada

doi:10.1248/bpb.25.529

Notes

Effect of Cationic Liposomes in an in Vitro Transcription and Translation System

Rieko Tachibana, Hideyoshi Harashima, Tatsuhiro Ishida, Yasuo Shinohara, Mami Hino, Hiroshi Terada, Yoshinobu Baba, Hiroshi Kiwada

Author information

Keywords: cationic liposome, transcription, rapid translation system (RTS500), gene delivery

JOURNAL FREE ACCESS

2002 Volume 25 Issue 4 Pages 529-531

DOI https://doi.org/10.1248/bpb.25.529

Details

Abstract

The effects of cationic liposomes complexed with plasmid DNA on the process of transcription was examined using a recently developed rapid cell free translation system. The findings indicate that the liposome itself inhibited the process when the ratio of DNA/liposome typically used in transfection studies was used.

Corresponding author

Register with J-STAGE for free!