Anthraquinones from Neonauclea calycina and Their Inhibitory Activity against DNA Topoisomerase II

Hideki TOSA; Munekazu IINUMA; Fujio ASAI; Toshiyuki TANAKA; Hiroshi NOZAKI; Shougo IKEDA; Ken TSUTSUI; Kimiko TSUTSUI; Masashi YAMADA; Shiho FUJIMORI

doi:10.1248/bpb.21.641

Hideki TOSA, Munekazu IINUMA, Fujio ASAI, Toshiyuki TANAKA, Hiroshi NOZAKI, Shougo IKEDA, Ken TSUTSUI, Kimiko TSUTSUI, Masashi YAMADA, Shiho FUJIMORI

Author information

Hideki TOSA
Munekazu IINUMA
Fujio ASAI
Toshiyuki TANAKA
Hiroshi NOZAKI
Shougo IKEDA
Ken TSUTSUI
Kimiko TSUTSUI
Masashi YAMADA
Shiho FUJIMORI
Author's Organization：
Department of Pharmacognosy, Gifu Pharmaceutical University
Department of Pharmacognosy, Gifu Pharmaceutical University
Department of Pharmacognosy, Gifu Pharmaceutical University
Department of Pharmacognosy, Gifu Pharmaceutical University
Department of Biological Chemistry, Faculty of Science, Okayama University of Science
Department of Biological Chemistry, Faculty of Science, Okayama University of Science
Department of Molecular Biology, Institute of Cellular and Molecular Biology, Medical School, Okayama University
Department of Molecular Biology, Institute of Cellular and Molecular Biology, Medical School, Okayama University
Meiji Milk Products Co., Ltd.,
Meiji Milk Products Co., Ltd.,

Keywords: Neonauclea calycina, Rubiaceae, anthraquinone, damnacanthal, morindone, topoisomerase II inhibitory activity

JOURNAL FREE ACCESS

1998 Volume 21 Issue 6 Pages 641-642

DOI https://doi.org/10.1248/bpb.21.641

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Abstract

In a series of searches fot DNA topoisomerase II inhibitors from naturally occurring compounds, a wood extract of Neonauclea calycina MERR. (Rubiaceae) showed a moderate effect in vitro. Purification of the extract resulted in the isolation of seven known anthraquinones. The structures were characterized as damnacanthal, rubiadin 1-methyl ether, nordamnacanthal, morindone, damnacanthol, lucidin 3-O-primeveroside and morindone 6-O-primeveroside by spectral analysis, respectively. Damnacanthal and morindone showed an intensive inhibitory effect against topoisomerase II (IC₅₀ : 20μg/ml and 21μg/ml).

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