Preparation of Monoclonal Antibodies for Immunoblotting Human Growth Hormone Receptor and Growth Hormone-Binding Protein

Yoshiro SAITO; Takeshi YAMAZAKI; Kazuhiro SUZUKI; Hideharu IKEBUCHI; Akira ASAKURA; Yoshimi OTA; Junichi SAWADA; Tadao TERAO

doi:10.1248/bpb.17.983

Yoshiro SAITO, Takeshi YAMAZAKI, Kazuhiro SUZUKI, Hideharu IKEBUCHI, Akira ASAKURA, Yoshimi OTA, Junichi SAWADA, Tadao TERAO

Author information

Yoshiro SAITO
Takeshi YAMAZAKI
Kazuhiro SUZUKI
Hideharu IKEBUCHI
Akira ASAKURA
Yoshimi OTA
Junichi SAWADA
Tadao TERAO
Author's Organization：
Division of Biochemistry and Immunochemistry, National Institute of Health Sciences
Division of Biochemistry and Immunochemistry, National Institute of Health Sciences
Division of Biochemistry and Immunochemistry, National Institute of Health Sciences:(Present adress)Division of Xenobiotic Metabolism and Disposition, National Institute of Health Sciences
Division of Biochemistry and Immunochemistry, National Institute of Health Sciences
Protein Engineering Research Institute:(Present adress)Nippon Roche Research Center
Protein Engineering Research Institute
Division of Biochemistry and Immunochemistry, National Institute of Health Sciences
Division of Biochemistry and Immunochemistry, National Institute of Health Sciences

Keywords: monoclonal antibody, immunoblotting, growth hormone receptor, growth hormone-binding protein

JOURNAL FREE ACCESS

1994 Volume 17 Issue 7 Pages 983-986

DOI https://doi.org/10.1248/bpb.17.983

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Abstract

Monoclonal anti-peptide antibodies against the extracellular domain of human growth hormone receptor (hGHR) were prepared. Four monoclonal antibodies (mAbs) reacted with an extracellular domain protein produced by genetic engineering. Among them, GHRP2-88 was the most reactive against hGHRs from human IM-9 cells. The lower limit of detection for immunoblotting using this mAb was about 200 pg hGHR. The GHRP2-88 antibody also reacted with deglycosylated hGHRs from tunicamycin-treated IM-9 cells and with the growth hormone-binding protein (GH-BP) in human plasma.

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