Pharmacological Activity of Chemically Modified Subfragment from Human Serum IgG. XIV. Inhibitory Effect of Carboxamide-Methylated Light Chain (G1L) on Tyrosine Phosphorylation and Tumor Necrosis Factor-α Production from Murine Macrophages Stimulated by Lipopolysaccharide

Tsutomu MIMURA; Katsuyoshi TAKEUCHI; Keiko HIRAMATSU; Kazutake TSUJIKAWA; Yasuhiro KOHAMA; Susumu ITOH

doi:10.1248/bpb.18.1377

Pharmacological Activity of Chemically Modified Subfragment from Human Serum IgG. XIV. Inhibitory Effect of Carboxamide-Methylated Light Chain (G₁L) on Tyrosine Phosphorylation and Tumor Necrosis Factor-α Production from Murine Macrophages Stimulated by Lipopolysaccharide

Tsutomu MIMURA, Katsuyoshi TAKEUCHI, Keiko HIRAMATSU, Kazutake TSUJIKAWA, Yasuhiro KOHAMA, Susumu ITOH

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Keywords: human serum IgG, carboxamide-methylated light chain, LPS, TNF-α, tyrosine phosphorylation

JOURNAL FREE ACCESS

1995 Volume 18 Issue 10 Pages 1377-1381

DOI https://doi.org/10.1248/bpb.18.1377

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Abstract

Carboxamide-methylated light chain (G₁L) from human serum IgG inhibited the secretion of tumor necrosis factor (TNF-α), one of the inflammatory cytokines, from adherent splenocytes and thioglycolate-induced peritoneal macrophages. The inhibition of TNF-α secretion by G₁L was associated with disappearance of tyrosine phosphorylation on about 40kDa protein when thioglycolate-induced peritoneal macrophages were stimulated with lipopolysaccharide (LPS). It is possible that this G₁L anti-inflammatory activity occurs through the blockage of the phosphorylation of about 40kDa protein.

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