南方医科大学学报

南方医科大学学报 ›› 2020, Vol. 40 ›› Issue (10): 1480-1487.doi: 10.12122/j.issn.1673-4254.2020.10.14

• • 上一篇    下一篇

双氢青蒿素通过调节肥大细胞浸润减轻小鼠特应性皮炎

  

  • 出版日期:2020-10-20 发布日期:2020-10-20

Dihydroartemisinin alleviates atopic dermatitis in mice by inhibiting mast cell infiltration

  • Online:2020-10-20 Published:2020-10-20

RichHTML

38

HTML

PDF

409

摘要： 目的 观察不同剂量的双氢青蒿素（DHA）对C57BL/6小鼠特应性皮炎（AD）的治疗作用及相关机制。方法 将42只C57BL/6小鼠随机分为7组，每组6只：空白对照组（无处理）、模型对照组（2,4-二硝基氯苯DNCB）、溶剂对照组（DNCB+溶解剂）、25 mg/kg DHA治疗组（DNCB+25 mg/kg DHA）、75 mg/kg DHA治疗组（DNCB+75 mg/kg DHA）、125 mg/kg DHA治疗组（DNCB+125 mg/kg DHA）和地塞米松治疗组（DNCB+地塞米松）。每天观测各组搔抓次数和皮损程度并进行评分。连续7 d给药后，将各组小鼠背部及耳部皮损涂抹处取材进行 HE 染色，Masson染色，甲苯胺蓝染色，观察皮肤病理学改变及炎症反应的好转情况。结果 25、75、125 mg/kg DHA治疗组，地塞米松治疗组均能缓解小鼠症状。小鼠皮损症状减轻，皮损评分降低，组织病理学改善，但125 mg/kg DHA治疗组效果最佳，能明显减少肥大细胞浸润，相对其余各组均具有统计学差异（P<0.05）。 结论 DHA对小鼠AD有较好的治疗效果，125 mg/kg DHA组治疗效果最佳。推测DHA可能通过抑制肥大细胞浸润，调节局部免疫，改善AD症状。

关键词: 特应性皮炎, 双氢青蒿素, 肥大细胞, 2,4-二硝基氯苯

Abstract: Objective To observe the therapeutic effect of different doses of dihydroartemisinin (DHA) on atopic dermatitis (AD) in mice and explore the mechanism. Methods Forty-two C57BL/6 mice were randomly divided into 7 groups (n=6), including a blank control group, a 2,4-dinitrochlorobenzene (DNCB)-induced AD model group, a solvent-treated group, 3 DHA treatment groups treated with 25, 75, and 125 mg/kg DHA, and a dexamethasone treatment group. The counts of skin scratches were recorded and the lesion scores were evaluated on a daily basis. After 7 consecutive days of treatment, skin tissues were sampled from the lesions on the back and ear of the mice for pathological examination with HE staining, Masson staining and toluidine blue staining. Results Treatment with 25, 75, and 125 mg/kg DHA and dexamethasone all alleviated AD symptoms of mice, reduced the severity scores of skin lesions, and ameliorated pathological changes of the skin tissue. DHA at 125 mg/kg produced the most obvious therapeutic effect and significantly alleviated mast cell infiltration in the lesions as compared with the other treatment groups (P<0.05). Conclusion DHA is effective for the treatment of AD in mice with an optimal dose of 125 mg/kg. The therapeutic effect of DHA is achieved probably through regulation of local immunity by inhibiting mast cell infiltration in the lesions.

Key words: atopic dermatitis, dihydroartemisinin, mast cells, 2,4-Dinitrochlorobenzene