南方医科大学学报

南方医科大学学报 ›› 2020, Vol. 40 ›› Issue (04): 567-572.doi: 10.12122/j.issn.1673-4254.2020.04.19

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METTL14在肝细胞癌中的表达及临床意义

周腾飞，任子夫，陈超爽   

  • 出版日期:2020-04-30 发布日期:2020-04-20
  • 基金资助:

METTL14 as a predictor of postoperative survival outcomes of patients with hepatocellular carcinoma

  

  • Online:2020-04-30 Published:2020-04-20

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摘要： 目的 探究RNA甲基转移酶METTL14在肝细胞癌（HCC）中的表达，及其临床意义。方法 采用免疫组织化学染色的方法，检测METTL14在147对肝癌、癌旁肝组织中的表达，根据病理医师的评分，把147例肝癌组织表达分为METTL14高表达组与METTL14低表达组，通过卡方检验分析其表达量高低与临床病理指标的相关性；应用Kaplan-Meier方法分析METTL14表达水平与肝癌患者术后预后生存的关系，包括总生存期与无瘤生存期；运用COX回归模型对METTL14表达与肝癌患者术后的总生存期与无瘤生存期进行单因素分析以及多因素分析，以探索METTL14的表达水平是否可作为肝癌患者术后预后的独立预后危险因素。结果 与癌旁组织相比，METTL14在HCC组织中表达明显降低（P<0.001）；HCC组织中METTL14表达与HCC患者肿瘤大小（P=0.044）及TNM分期（P=0.046）相关；HCC组织中METTL14的低表达与患者的不良预后显著相关，其术后总生存期及无瘤生存期明显缩短（P<0.05），且是影响HCC患者术后总生存期及无瘤生存期的一个独立危险因素。结论 METTL14可能成为一种原发性肝癌切除术后的新的预后指标。

Abstract: Objective To investigate the expression of RNA methyltransferase METTL14 in hepatocellular carcinoma (HCC) and its clinical significance. Methods Immunohistochemical staining was used to detect the expression of METTL14 in 147 pairs of HCC and adjacent hepatic tissues. According to the scores rated by pathologists, the 147 cases of HCC were divided into high and low METTL14 expression groups. The correlation between the expression of METTL14 and clinicopathological parameters was analyzed, and Kaplan-Meier method was used to analyze the relationship between the expression of METTL14 and the prognosis and survival (including the overall survival and disease-free survival) of the patients with HCC after operation. Univariate analysis and multivariate analysis were carried out to assess the impact of METTL14 expression level on the overall survival and tumor-free survival of the patients after operation using a COX regression model and explore whether METTL14 expression level is an independent prognostic risk factor of the postoperative patients. Results The expression of METTL14 was significantly lower in HCC tissues than in the adjacent tissues (P<0.001). METTL14 expression in HCC tissues was significantly correlated with the tumor size (P=0.044) and TNM stage (P=0.046). A low expression of METTL14 in HCC tissues was significantly correlated with a poor prognosis and a significantly shortened overall survival time and disease-free survival time of the patients (P<0.05), and was an independent risk factor affecting the overall survival and disease-free survival of HCC patients. Conclusion METTL14 may be a new prognostic marker for patients with HCC after hepatectomy.