法医学杂志

法医学杂志 ›› 2022, Vol. 38 ›› Issue (6): 739-746.DOI: 10.12116/j.issn.1004-5619.2021.511102

• 技术与应用 • 上一篇    下一篇

北川羌族人群57个常染色体InDel位点的遗传多态性及群体遗传结构分析

蒋春月1(), 马浩1, 范庆炜1, 杨慧凌1, 徐冬冬1, 王韵1, 杜冰1,2()   

  1. 1.川北医学院基础医学与法医学院，四川 南充 637000
    2.川北医学院司法鉴定中心，四川 南充 637000
  • 收稿日期:2021-11-01 发布日期:2022-12-25 出版日期:2022-12-28
  • 通讯作者: 杜冰
  • 作者简介:杜冰，女，博士，教授，主要从事法医学教学、科研和鉴定；E-mail：dbing04@163.com
    蒋春月（1996—），女，硕士研究生，主要从事法医物证学研究；E-mail：Jcy_nsmc@163.com
  • 基金资助:
    南充市科学技术和知识产权局科研项目(16YFZJ0131)

Analysis of Genetic Polymorphism and Population Genetic Structure of 57 Autosomal InDel Loci in Beichuan Qiang Population

Chun-yue JIANG1(), Hao MA1, Qing-wei FAN1, Hui-ling YANG1, Dong-dong XU1, Yun WANG1, Bing DU1,2()   

  1. 1.School of Basic Medicine and Forensic Medicine, North Sichuan Medical College, Nanchong 637000, Sichuan Province, China
    2.Forensic Science Service Center of North Sichuan Medical College, Nanchong 637000, Sichuan Province, China
  • Received:2021-11-01 Online:2022-12-25 Published:2022-12-28
  • Contact: Bing DU

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摘要：

目的 调查AGCU InDel 60荧光检测试剂盒中57个常染色体InDel（autosomal-InDel，A-InDel）位点在四川省北川羌族人群中的遗传学信息，并评估其法医学应用价值。 方法 采用AGCU InDel 60荧光检测试剂盒对北川200名羌族健康无关个体进行分型检测，统计分析57个A-InDel位点的等位基因频率和群体遗传学参数，并与已知的26个人群数据进行比较。 结果 经Bonferroni法校正，57个A-InDel位点之间不存在连锁不平衡现象，且所有位点均符合Hardy-Weinberg平衡。除rs66595817、rs72085595外，其他55个A-InDel位点的最小等位基因频率均大于0.3。PIC为0.298 3~0.375 0，CDP为1-2.974 8×10-24，CPEduo为0.999 062 660，CPEtrio为0.999 999 999。计算遗传距离发现，北川羌族人群与北京汉族和南方汉族人群遗传距离最近，与非洲人群遗传距离较远。 结论 AGCU InDel 60荧光检测试剂盒所包含的57个A-InDel位点在四川省北川羌族人群中具有良好的遗传多态性，可作为法医学个体识别和亲权鉴定的有效补充。

关键词: 法医遗传学, 遗传多态性, 插入/缺失, 常染色体, 遗传距离, 羌族, 北川

Abstract:

Objective To investigate the genetic information of 57 autosomal InDel loci （A-InDels） included in AGCU InDel 60 fluorescence detection kit in the Beichuan Qiang population of Sichuan Province and evaluate its application value in forensic medicine. Methods A total of 200 unrelated healthy individuals from Beichuan Qiang population of Sichuan Province were typing detected by AGCU InDel 60 fluorescence detection kit. Allele frequencies and population genetic parameters of the 57 A-InDels were statistically analyzed and compared with the available data of 26 populations. Results After Bonferroni correction, there was no linkage disequilibrium between the 57 A-InDels, and all loci were in Hardy-Weinberg equilibrium. Except for rs66595817 and rs72085595, the minor allele frequencies of 55 A-InDels were above 0.3. PIC ranged from 0.298 3 to 0.375 0, CDP was 1-2.974 8×10-24, CPEduo was 0.999 062 660, and CPEtrio was 0.999 999 999. The calculation of the genetic distance showed that Beichuan Qiang population had the closest genetic distances with Beijing Han and South China Han populations, but far away from African populations. Conclusion The 57 A-InDels in AGCU InDel 60 fluorescence detection kit have a good genetic polymorphism in Beichuan Qiang population of Sichuan Province, which can be used as effective supplemental for individual identification and paternity identification in forensic medicine.

Key words: forensic genetics, genetic polymorphism, insertion/deletion （InDel）, autosome, genetic distance, Qiang ethnic group, Beichuan

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