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Mupirocin-Loaded Chitosan Microspheres Embedded in Piper betle Extract Containing Collagen Scaffold Accelerate Wound Healing Activity

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Abstract

This study reports the formulation of mupirocin-loaded chitosan microspheres embedded in Piper betle extract containing collagen scaffold as combinational drug delivery for improved wound healing. Selection of chitosan type (molecular weight and degree of deacetylation) was carried out based on their antibacterial efficacy. The low molecular weight chitosan was selected owing to the highest antibacterial action against gram-positive as well as gram-negative bacteria. Low molecular weight chitosan-microspheres showed spherical shape with largely smooth surface morphology, 11.81% of mupirocin loading, and its controlled release profile. The XRD, DSC thermograms, and FT-IR spectral analysis revealed the mupirocin loaded in molecularly dispersed or in amorphous form, and having no chemical interactions with the chitosan matrix, respectively. The in vivo study indicates potential effect of the mupirocin, Piper betle, and chitosan in the collagen scaffold in the wound healing efficiency with approximately 90% wound healing observed at the end of 15 days of study for combinational drug-loaded chitosan microspheres-collagen scaffold-treated group. The histopathology examination further revealed tissue lined by stratified squamous epithelium, collagen deposition, fibroblastic proliferation, and absence of inflammation indicating relatively efficient wound healing once treated with combinational drug-loaded chitosan microspheres containing scaffold.

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Funding

The support for this study is provided by Taif University Researchers Supporting Project Number (TURSP-2020/33), Taif University, Saudi Arabia.

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Author notes

  1. Sohail Akhter

    Present address: New Product Development, Global R&D, Sterile Ops, TEVA Pharmaceutical Industries Ltd., Aston Ln N, Halton, Preston Brook, Runcorn, WA7 3FA, UK

  2. Mansi Budhiraja, Sobiya Zafar, Sohail Akhter and Sarwar Beg contributed equally to this work.

Authors and Affiliations

  1. Nanomedicine Research Lab, School of Pharmaceutical Education and Research, Jamia Hamdard, New Delhi, India

    Mansi Budhiraja, Sobiya Zafar, Sohail Akhter, Sarwar Beg & Farhan J. Ahmad

  2. Nucleic Acids Transfer by Non-Viral Methods, Centre de Biophysique Moléculaire, CNRS UPR4301, Rue Charles Sadron, 45071, Orléans Cedex 2, France

    Sohail Akhter

  3. LE STUDIUM® Loire Valley Institute for Advanced Studies, Centre-Val de Loire Region, France

    Sohail Akhter

  4. Faculty of Medicine, Yousef Abdullatif Jameel Chair of Prophetic Medicine Application (YAJCPMA), King Abdulaziz University Hospital (KAUH), King Abdulaziz University (KAU), Jeddah, 21589, Saudi Arabia

    Sohail Akhter

  5. Department of Pharmaceutics and Industrial Pharmacy, College of Pharmacy, Taif University, Taif, Saudi Arabia

    Majed Alrobaian

  6. Department of Pharmaceutics, College of Pharmacy, King Khalid University, Abha, Aseer, 62529, Saudi Arabia

    Md Abdur Rashid

  7. Department of Pharmaceutics, College of Pharmacy, University of Hafr Al Batin, Hafr Al Batin, Saudi Arabia

    Md. Abul Barkat

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  1. Mansi Budhiraja
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Budhiraja, M., Zafar, S., Akhter, S. et al. Mupirocin-Loaded Chitosan Microspheres Embedded in Piper betle Extract Containing Collagen Scaffold Accelerate Wound Healing Activity. AAPS PharmSciTech 23, 77 (2022). https://doi.org/10.1208/s12249-022-02233-9

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