Skip to main content
SpringerLink
Log in

A Comparison of Pregnancy Outcomes in Patients Undergoing Donor Egg Single Embryo Transfers With and Without Preimplantation Genetic Testing

  • Published:
Reproductive Sciences Aims and scope Submit manuscript

Abstract

Two of the many milestone developments in the field of assisted reproduction have been oocyte donation and preimplantation genetic testing for aneuploidy (PGT-A). Because it has been demonstrated that even young women produce a meaningful proportion of aneuploid embryos, screening out such abnormalities could potentially increase the efficacy of donor egg (DE) cycles. In this retrospective cohort study, we investigated the effect of PGT-A on DE cycle outcomes, including implantation rate (IR), spontaneous abortion rate (SABR), and ongoing pregnancy/live birth rate. We used fresh and frozen donor cycles not using PGT-A as comparison groups; all cases involved single embryo transfer. Data analysis revealed that PGT-A did not improve pregnancy outcome metrics in DE cycles, although there was a trend toward decreasing the SABR. There was a significant increase in IR with fresh cycles outperforming all frozen cycles. Overall, these results suggest that the benefits of performing PGT-A on embryos derived from young DEs may be limited and that there is an effect of the freezing process on pregnancy outcomes. These findings may provide useful insights into the science and practice of PGT-A across all of its applications.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Sauer MV, Paulson RJ. Human oocyte and preembryo donation: an evolving method for the treatment of infertility. Am J Obstet Gynecol. 1990;163(5 pt 1):1421–1424.

    Article  CAS  Google Scholar 

  2. Handyside AH, Kontogianni EH, Hardy K, et al. Pregnancies from biopsied human preimplantation embryos sexed by Y-specific DNA amplification. Nature. 1990;344(6268):768–770.

    Article  CAS  Google Scholar 

  3. Lee HL, McCulloh DH, Hodes-Wertz B, et al. Invitro fertilization with preimplantation genetic screening improves implantation and live birth in women age 40 through 43. J Assist Reprod Genet. 2015;32(3):435–444.

    Article  Google Scholar 

  4. Baart EB, Martini E, van den Berg I, et al. Preimplantation genetic screening reveals a high incidence of aneuploidy and mosaicism in embryos from young women undergoing IVF. Hum Reprod. 2006;21(1):223–233.

    Article  CAS  Google Scholar 

  5. Sills ES, Li X, Frederick JL, et al. Determining parental origin of embryo aneuploidy: analysis of genetic error observed in 305 embryos derived from anonymous donor oocyte IVF cycles. Mol Cytogenet. 2014;7(1):68.

    Article  Google Scholar 

  6. Franasiak JM, Forman EJ, Hong KH, et al. The nature of aneuploidy with increasing age of the female partner: a review of 15,169 consecutive trophectoderm biopsies evaluated with comprehensive chromosomal screening. Fertil Steril. 2014;101(3):656–663.

    Article  Google Scholar 

  7. Yang Z, Liu J, Collins GS, et al. Selectionofsingle blastocysts for fresh transfer via standard morphology assessment alone and with array CGH for good prognosis IVF patients: results from a randomized pilot study. Mol Cytogenet. 2012;2:5(1):24.

    Article  Google Scholar 

  8. Forman EJ, Tao X, Ferry KM, et al. Single embryo transfer with comprehensive chromosome screening results in improved ongoing pregnancy rates and decreased miscarriage rates. Hum Reprod. 2012;27(4):1217–1222

    Article  CAS  Google Scholar 

  9. Fedorova EM, Shlykova SA, Shunkina KV, et al. Outcomes of IVF cycles coupled with PGT by aCGH of embryos from donor and autologous oocytes, transferred after vitrification to women of advanced maternal age. Gynecol Endocrinol. 2017;33(9): 737–740

    Article  Google Scholar 

  10. Haddad G, Deng M, Wang CT, et al. Assessment of aneuploidy formation in human blastocysts resulting from donated eggs and the necessity of the embryos for aneuploidy screening. J Assist Reprod Genet. 2015;32(6):999–1006.

    Article  Google Scholar 

  11. Barad DH, Darmon SK, Kushnir VA, et al. Impact of preimplantation genetic screening on donor oocyte-recipient cycles in the United States. Obstet Gynecol. 2017;217(5):576.e1–576.e8. pii: S0002-9378(17)30865-7. doi: 10.1016/j.ajog.2017.07.023.

    Google Scholar 

  12. Munne S, Alikani M, Ribustello L, Colls P, Martinez-Ortiz PA, McCulloh DH; Referring Physician Group. Euploidy rates in donor egg cycles significantly differ between fertility centers. Hum Reprod. 2017;32(4):743–749.

    Article  CAS  Google Scholar 

  13. Fragouli E, Escalona A, Gutierrez-Mateo C, et al. Comparative genomic hybridization of oocytes and first polar bodies from young donors. Reprod Biomed Online. 2009;19(2):228–237.

    Article  CAS  Google Scholar 

  14. Kuliev A, Cieslak J, Ilkevitch Y, et al. Chromosomal abnormalities in a series of 6,733 human oocytes in preimplantation diagnosis for age-related aneuploidies. Reprod Biomed Online. 2003;6(1):54–59.

    Article  Google Scholar 

  15. Maxwell SM, Colls P, Hodes-Wertz B, et al. Why do euploid embryos miscarry? A case-control study comparing the rate of aneuploidy within presumed euploid embryos that resulted in miscarriage or live birth using next-generation sequencing. Fertil Steril. 2016;106(6):1414–1419.e5.

    Article  CAS  Google Scholar 

  16. Greco E, Minasi MG, Fiorentino F. Healthy babies after intrauterine transfer of mosaic aneuploid blastocysts. N Engl J Med. 2015;373(21):2089–2090.

    Article  Google Scholar 

  17. Sachdev NM, Maxwell SM, Besser AG, Grifo JA. Diagnosis and clinical management ofembryonic mosaicism. Fertil Steril. 2017;107(1):6–11.

    Article  Google Scholar 

  18. Gutierrez-Mateo C, Colls P, Sanchez-Garcia J, et al. Validationof microarray comparative genomic hybridization for comprehensive chromosome analysis of embryos. Fertil Steril. 2011;95(3):953–958.

    Article  CAS  Google Scholar 

  19. Fiorentino F, Biricik A, Bono S, et al. Development and validation of a next-generation sequencing-based protocol for 24-chromosome aneuploidy screening of embryos. Fertil Steril. 2014;101(5):1375–1382.

    Article  CAS  Google Scholar 

  20. Keefe DL. Telomeres, reproductive aging, and genomic instability during early development. Reprod Sci. 2016;23(12):1612–1615.

    Article  CAS  Google Scholar 

  21. Ge SX. Exploratory bioinformatics investigation reveals importance of “junk” DNA in early embryo development. BMC Genomics. 2017;18(1):200.

    Article  Google Scholar 

  22. Guo H, Zhu P, Yan L, et al. The DNA methylation landscape of human early embryos. Nature. 2014;511(7511):606–610.

    Article  CAS  Google Scholar 

  23. Scott RT Jr. Upham KM, Forman EJ, Zhao T, Treff NR. Cleavage-stage biopsy significantly impairs human embryonic implantation potential while blastocyst biopsy does not: a randomized and paired clinical trial. Fertil Steril. 2013;100(3):624–630.

    Article  Google Scholar 

  24. Barrie A, Homburg R, McDowell G, Brown J, Kingsland C, Troup S. Preliminary investigation ofthe prevalence and implantation potential of abnormal embryonic phenotypes assessed using time-lapse imaging. Reprod Biomed Online. 2017;34(5):455–462.

    Article  Google Scholar 

  25. Sole M, Santalo J, Boada M, et al. How does vitrification affect oocyte variability in oocyte donation cycles? A prospective study to compare outcomes achieved with fresh versus vitrified sibling oocytes. Hum Reprod. 2013;28(8):2087–2092.

    Article  CAS  Google Scholar 

  26. Coates A, Bankowski BJ, Kung A, Griffin DK, Munne S. Differences in pregnancy outcomes in donor egg frozen embryo transfer (FET) cycles following preimplantation genetic screening (PGS): a single center retrospective study. J Assist Reprod Genet. 2017; 34:71–78.

    Article  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. NYU Fertility Center, 660 First Avenue, Fifth Floor, New York, NY, 10016, USA

    Alexis K. Masbou (MD), David H. McCulloh (PhD, HCLD), Caroline McCaffrey (PhD, HCLD), M. Elizabeth Fino (MD), James A. Grifo (MD, PhD) & Frederick Licciardi (MD)

  2. Obstetrics and Gynecology, NYU Langone Medical Center, New York, NY, USA

    Jenna B. Friedenthal (MD)

Authors
  1. Alexis K. Masbou
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Jenna B. Friedenthal
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. David H. McCulloh
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Caroline McCaffrey
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. M. Elizabeth Fino
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. James A. Grifo
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Frederick Licciardi
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Alexis K. Masbou.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Masbou, A.K., Friedenthal, J.B., McCulloh, D.H. et al. A Comparison of Pregnancy Outcomes in Patients Undergoing Donor Egg Single Embryo Transfers With and Without Preimplantation Genetic Testing. Reprod. Sci. 26, 1661–1665 (2019). https://doi.org/10.1177/1933719118820474

Download citation

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1177/1933719118820474

Keywords

Search

Navigation