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Overexpression of Collapsin Response Mediator Protein 1 Inhibits Human Trophoblast Cells Proliferation, Migration, and Invasion

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Abstract

Collapsin response mediator protein 1 (CRMP-1) is widely expressed in the nervous system and has tumor suppressive effects. Our previous studies have demonstrated that CRMP-1 was expressed in the trophoblasts of the whole stage of pregnancy with significantly increasing expression in the placenta of early-onset preeclampsia. Preeclampsia, especially early onset, is strongly associated with the dysfunction of trophoblast including proliferation, apoptosis, migration, and invasion. In this study, we found an inhibitory effect of CRMP-1 on proliferation, migration, invasion, and an enhanced effect on apoptosis in human trophoblast cell lines HTR-8/SVneo and JEG-3 by MTT assay, colony formation assay, cell viability assay, caspase 3/7 activity assay, scratch wound assay, and Matrigel Transwell assay. Overexpression of CRMP-1 in trophoblast cells led to downregulate expression of matrix metalloproteinase 2 and 9. The expression of CRMP-1 was detected by real-time quantitative polymerase chain reaction and Western blot analysis. Thus, we suggested that CRMP-1 might have implications for the pathogenesis of preeclampsia by regulating the biological behavior of trophoblast cells.

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Author notes

  1. Ling Huang and Yuanyuan Li contributed equally to this work.

Authors and Affiliations

  1. Department of Obstetrics and Gynecology, Shengjing Hospital, China Medical University, No.36, Sanhao street, Shenyang, Liaoning Province, 110004, China

    Ling Huang MD, Yuanyuan Li MD, Na Li MD, Yue Hou MD, Man Sun MD & Chong Qiao MD, PhD

  2. Key Laboratory of Maternal-Fetal Medicine, China Medical University, Shenyang, Liaoning Province, China

    Ling Huang MD, Yuanyuan Li MD, Na Li MD, Yue Hou MD, Man Sun MD & Chong Qiao MD, PhD

  3. Key Laboratory of Obstetrics and Gynecology of Higher Education, China Medical University, Shenyang, Liaoning Province, China

    Ling Huang MD, Yuanyuan Li MD, Na Li MD, Yue Hou MD, Man Sun MD & Chong Qiao MD, PhD

  4. Department of Hepatobiliary Surgery, General Hospital of Shenyang Military Region, Shenyang, Liaoning Province, China

    Chunhui Wang MD, PhD

  5. Department of Obstetrics and Gynecology, Shenyang Forth People’s Hospital, Shenyang, Liaoning Province, China

    Na Li MD

  6. Department of Obstetrics and Gynecology, The Second Hospital of Hebei Medical University, Shijiazhuang, Hebei, China

    Ruijing Chang MD

  7. Department of Biomedical Engineering, University of Wisconsin at Madison, Madison, WI, USA

    Ruochen Wang

  8. Shenyang Academy of Environmental Sciences, Shenyang, Liaoning Province, China

    Lei Zhu MD

Authors
  1. Ling Huang MD
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  2. Yuanyuan Li MD
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  3. Chunhui Wang MD, PhD
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  4. Na Li MD
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  5. Yue Hou MD
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  7. Man Sun MD
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  8. Ruochen Wang
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  9. Lei Zhu MD
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  10. Chong Qiao MD, PhD
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Corresponding author

Correspondence to Chong Qiao MD, PhD.

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Huang, L., Li, Y., Wang, C. et al. Overexpression of Collapsin Response Mediator Protein 1 Inhibits Human Trophoblast Cells Proliferation, Migration, and Invasion. Reprod. Sci. 26, 954–960 (2019). https://doi.org/10.1177/1933719118799214

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