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Effects of Urinary and Recombinant Gonadotropins on In Vitro Maturation Outcomes of Mouse Preantral Follicles

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Abstract

Gonadotropins including follicle-stimulating hormone (FSH) and luteinizing hormone (LH) play a crucial role in human-assisted reproduction techniques. Despite wide use of recombinant gonadotropins in clinical practice, the efficacy of urinary gonadotropins and the dosage of LH component have not yet been elucidated. This study was designed to investigate the difference of follicle culture outcomes according to various compositions of gonadotropins during in vitro culture of mouse preantral follicles. Ovaries were obtained from the 14-day-old C57BL/6 mice, and preantral follicles were isolated and cultured in culture media supplemented with human menopausal gonadotropin (hMG) 200 mIU/mL (group 1), recombinant FSH and LH (rFSH + rLH) 200 mIU/mL each (group 2), rFSH 200 mIU/mL + rLH 100 mIU/mL (group 3), or rFSH 200 mIU/mL + rLH 20 mIU/mL (group 4). Follicle survival rate was significantly lower in group 4. Antral follicles in lower doses of LH (groups 3, 4) showed a statistically significant larger diameter and tended to have a higher antral formation rate. However, follicles in group 1 tended to have a higher oocyte maturation rate. Estradiol concentration from conditioned media from 2:1 FSH/LH (group 3) was significantly higher than those from 1:1 FSH/LH (group 2) or 10:1 FSH/LH (group 4). Half dose of rLH to rFSH facilitated upregulation of growth differentiation factor 9 (Gdf9) expression in granulosa cells when compared to 1:1 FSH/LH or 10:1 FSH/LH. Conclusively, recombinant gonadotropins provided a comparable condition to hMG, and half dose of rLH to rFSH seems to be more suitable for follicular development during in vitro culture.

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Authors and Affiliations

  1. Department of Obstetrics and Gynecology, College of Medicine, Seoul National University Hospital, 101 Daehak-Ro, Jongno-Gu, 110-744, Seoul, South Korea

    Kyung Eui Park MD, Seung-Yup Ku MD, PhD, Seok Hyun Kim MD, PhD, Young Min Choi MD, PhD, Jung Gu Kim MD, PhD & Shin Yong Moon MD, PhD

  2. Institute of Reproductive Medicine and Population, Medical Research Center, Seoul, South Korea

    Seung-Yup Ku MD, PhD, Yoon Young Kim PhD, Yong Jin Kim MD, Seok Hyun Kim MD, PhD, Young Min Choi MD, PhD & Shin Yong Moon MD, PhD

  3. Department of Pathology, College of Medicine, Seoul National University, Seoul, South Korea

    Kyung Cheon Jung MD, PhD

  4. Center for Reproductive Medicine and Infertility, Weill Cornell Medical College, New York, NY, USA

    Hung Ching Liu PhD

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  1. Kyung Eui Park MD
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  2. Seung-Yup Ku MD, PhD
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  9. Jung Gu Kim MD, PhD
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Correspondence to Seung-Yup Ku MD, PhD.

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Park, K.E., Ku, SY., Jung, K.C. et al. Effects of Urinary and Recombinant Gonadotropins on In Vitro Maturation Outcomes of Mouse Preantral Follicles. Reprod. Sci. 20, 909–916 (2013). https://doi.org/10.1177/1933719112468948

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