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Cyclic Regulation of T-Bet and GATA-3 in Human Endometrium

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Abstract

Endometrial cytokine expression is poorly understood. T-Bet and GATA-3 regulate cytokine expression in T-lymphocytes. Previous work has demonstrated expression of T-Bet in human endometrium. Changes in human endometrial T-Bet and GATA-3 mRNA and protein expression during the normal menstrual cycle were characterized. Human endometrium from each phase of the menstrual cycle underwent real-time reverse-transcriptase polymerase chain reaction and immunohistochemistry to examine expression and localization. T-Bet and GATA-3 mRNA were increased in the late secretory phase. Progesterone receptor (PR) mRNA was increased during the proliferative and early secretory phases. T-Bet and GATA-3 proteins localized cytoplasmically in the late secretory phase. PR protein displayed nuclear localization and maximal immunostaining during the early secretory phase. T-Bet and GATA-3 are expressed in endometrial epithelium cyclically during the menstrual cycle. T-Bet and GATA-3 are both upregulated during the late secretory phase and in the same cell types. The expression patterns of T-Bet and GATA-3 oppose PR, suggesting antagonistic function and/or regulation between PR and T-Bet/GATA-3.

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Authors and Affiliations

  1. Department of Obstetrics & Gynecology, University of North Carolina at Chapel Hill, CB# 7570, 4001 Old Clinic Bldg, Chapel Hill, NC, 27599, USA

    Danielle Inman & Steven Young MD, PhD

  2. School of Medicine, University of Tokyo, Tokyo, Japan

    Kei Kawana MD

  3. Department of Obstetrics & Gynecology, University of Missouri, Columbia, USA

    Danny Schust MD

  4. Department of Pathology, University of North Carolina at Chapel Hill, Chapel Hill, USA

    Ruth Lininger MD

Authors
  1. Danielle Inman
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  2. Kei Kawana MD
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  3. Danny Schust MD
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  4. Ruth Lininger MD
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  5. Steven Young MD, PhD
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Corresponding author

Correspondence to Danielle Inman.

Additional information

We gratefully acknowledge the financial support of the Holderness Foundation and the University of North Carolina Nova Carta Foundation.This research was also supported by the National Institute of Child Health and Human Development/National Institutes of Health through cooperative agreement U54 HD035041 as part of the Specialized Cooperative Centers Program in Reproduction and Infertility Research.We also gratefully acknowledge the outstanding technical assistance of Lingwen Yuan, PhD, Xi Yang, and Gail Grossman.

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Inman, D., Kawana, K., Schust, D. et al. Cyclic Regulation of T-Bet and GATA-3 in Human Endometrium. Reprod. Sci. 15, 83–90 (2008). https://doi.org/10.1177/1933719107309690

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