Natural Substances as Substrates in Metallic Nanoparticles Synthesis

The primary objective of nanoscience is to apply the unique properties of nanomaterials by control at the atomic and molecular level and to develop an effective manner for their preparation and use. The use of natural isolates for the synthesis of nanoparticles has many advantages, including availability of biological material, mild synthesis conditions and the lack of by-products. The main aim of the research is to investigate the possibility of bee products applying as innovative reductive system for metallic nanoparticles synthesis. Nanosilver was prepared by reaction of 250 ppm AgNO₃ solution in 3% PVP and aqueous extract from bee pollen. Bee pollen grains were characterized by scanning electron microscope (SEM), Fourier Transform Infrared Spectroscopy (FT-IR) and X-ray Fluorescence (XRF). The formation of silver nanoparticles in the suspension was monitored by UV-Vis spectrophotometer analysis. The diameter of particles size was characterized by Dynamic Light Scattering (DLS) and the tendency to agglomeration by scanning electron microscope (SEM).