Introgression the SALTOL QTL into Q5DB, the Elite Variety of Vietnam Using Marker- Assisted - Selection (MAS)

Luu Thi Ngoc Huyen; Luu Minh Cuc; Le Huy Ham; Tran Dang Khanh

doi:doi:10.11648/j.ajbio.20130104.15

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Introgression the SALTOL QTL into Q5DB, the Elite Variety of Vietnam Using Marker- Assisted - Selection (MAS)

Luu Thi Ngoc Huyen, Luu Minh Cuc, Le Huy Ham, Tran Dang Khanh

Published in American Journal of BioScience (Volume 1, Issue 4)

Received: 1 October 2013 Accepted: Published: 30 November 2013

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Abstract

Rice is a main food crop in Vietnam and plays a key role in economic growth in this country. However, its production and cultivating areas are severely affected from the threats of devastation caused by the rise of sea level. In this study, we have focused on developing new rice lines with salinity tolerance and high yield by applying markers assisted selection (MAS). Total of 21 primers in the Saltol QTL region were checked with the two parents varieties to identify polymorphic primers for screening the Saltol QTL region of the breeding populations. The individual plants in BC1, BC2, and BC3 generations of the Q5DB/FL478 were analyzed to evaluate the introgression of Saltol fragment into Q5DB cultivar. After screening of 3 BC generations, the best individual plants of BC3F1 of the plant numbers QF-3-1, QF-3-2, QF4-3-3, QF6-3 with almost the recipient alleles were selected. Conventional breeding was developed on BC3F2 for selection of the new salt tolerance rice lines with high yield, resistance to the biotic stress. The salinity tolerance of the new lines was also performed using the standard system of screening.

Published in	American Journal of BioScience (Volume 1, Issue 4)
DOI	10.11648/j.ajbio.20130104.15
Page(s)	80-84
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Q5DB, Marker Assisted Selection, Rice, Saltol- QTL

References

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APA Style

Luu Thi Ngoc Huyen, Luu Minh Cuc, Le Huy Ham, Tran Dang Khanh. (2013). Introgression the SALTOL QTL into Q5DB, the Elite Variety of Vietnam Using Marker- Assisted - Selection (MAS). American Journal of BioScience, 1(4), 80-84. https://doi.org/10.11648/j.ajbio.20130104.15

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ACS Style

Luu Thi Ngoc Huyen; Luu Minh Cuc; Le Huy Ham; Tran Dang Khanh. Introgression the SALTOL QTL into Q5DB, the Elite Variety of Vietnam Using Marker- Assisted - Selection (MAS). Am. J. BioScience 2013, 1(4), 80-84. doi: 10.11648/j.ajbio.20130104.15

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AMA Style

Luu Thi Ngoc Huyen, Luu Minh Cuc, Le Huy Ham, Tran Dang Khanh. Introgression the SALTOL QTL into Q5DB, the Elite Variety of Vietnam Using Marker- Assisted - Selection (MAS). Am J BioScience. 2013;1(4):80-84. doi: 10.11648/j.ajbio.20130104.15

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@article{10.11648/j.ajbio.20130104.15,
  author = {Luu Thi Ngoc Huyen and Luu Minh Cuc and Le Huy Ham and Tran Dang Khanh},
  title = {Introgression the SALTOL QTL into Q5DB, the Elite Variety of Vietnam Using Marker- Assisted - Selection (MAS)},
  journal = {American Journal of BioScience},
  volume = {1},
  number = {4},
  pages = {80-84},
  doi = {10.11648/j.ajbio.20130104.15},
  url = {https://doi.org/10.11648/j.ajbio.20130104.15},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajbio.20130104.15},
  abstract = {Rice is a main food crop in Vietnam and plays a key role in economic growth in this country. However, its production and cultivating areas are severely affected from the threats of devastation caused by the rise of sea level. In this study, we have focused on developing new rice lines with salinity tolerance and high yield by applying markers assisted selection (MAS). Total of 21 primers in the Saltol QTL region were checked with the two parents varieties to identify polymorphic primers for screening the Saltol QTL region of the breeding populations. The individual plants in BC1, BC2, and BC3 generations of the Q5DB/FL478 were analyzed to evaluate the introgression of Saltol fragment into Q5DB cultivar. After screening of 3 BC generations, the best individual plants of BC3F1 of the plant numbers QF-3-1, QF-3-2, QF4-3-3, QF6-3 with almost the recipient alleles were selected. Conventional breeding was developed on BC3F2 for selection of the new salt tolerance rice lines with high yield, resistance to the biotic stress. The salinity tolerance of the new lines was also performed using the standard system of screening.},
 year = {2013}
}

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TY  - JOUR
T1  - Introgression the SALTOL QTL into Q5DB, the Elite Variety of Vietnam Using Marker- Assisted - Selection (MAS)
AU  - Luu Thi Ngoc Huyen
AU  - Luu Minh Cuc
AU  - Le Huy Ham
AU  - Tran Dang Khanh
Y1  - 2013/11/30
PY  - 2013
N1  - https://doi.org/10.11648/j.ajbio.20130104.15
DO  - 10.11648/j.ajbio.20130104.15
T2  - American Journal of BioScience
JF  - American Journal of BioScience
JO  - American Journal of BioScience
SP  - 80
EP  - 84
PB  - Science Publishing Group
SN  - 2330-0167
UR  - https://doi.org/10.11648/j.ajbio.20130104.15
AB  - Rice is a main food crop in Vietnam and plays a key role in economic growth in this country. However, its production and cultivating areas are severely affected from the threats of devastation caused by the rise of sea level. In this study, we have focused on developing new rice lines with salinity tolerance and high yield by applying markers assisted selection (MAS). Total of 21 primers in the Saltol QTL region were checked with the two parents varieties to identify polymorphic primers for screening the Saltol QTL region of the breeding populations. The individual plants in BC1, BC2, and BC3 generations of the Q5DB/FL478 were analyzed to evaluate the introgression of Saltol fragment into Q5DB cultivar. After screening of 3 BC generations, the best individual plants of BC3F1 of the plant numbers QF-3-1, QF-3-2, QF4-3-3, QF6-3 with almost the recipient alleles were selected. Conventional breeding was developed on BC3F2 for selection of the new salt tolerance rice lines with high yield, resistance to the biotic stress. The salinity tolerance of the new lines was also performed using the standard system of screening.
VL  - 1
IS  - 4
ER  -

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Author Information

Luu Thi Ngoc Huyen

Molecular Biology Division, Agricultural Genetics Institute, Pham Van Dong Road, Tuliem, Hanoi, Vietnam
Luu Minh Cuc

Molecular Biology Division, Agricultural Genetics Institute, Pham Van Dong Road, Tuliem, Hanoi, Vietnam
Le Huy Ham

Molecular Biology Division, Agricultural Genetics Institute, Pham Van Dong Road, Tuliem, Hanoi, Vietnam
Tran Dang Khanh

Molecular Biology Division, Agricultural Genetics Institute, Pham Van Dong Road, Tuliem, Hanoi, Vietnam

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APA Style

Luu Thi Ngoc Huyen, Luu Minh Cuc, Le Huy Ham, Tran Dang Khanh. (2013). Introgression the SALTOL QTL into Q5DB, the Elite Variety of Vietnam Using Marker- Assisted - Selection (MAS). American Journal of BioScience, 1(4), 80-84. https://doi.org/10.11648/j.ajbio.20130104.15

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ACS Style

Luu Thi Ngoc Huyen; Luu Minh Cuc; Le Huy Ham; Tran Dang Khanh. Introgression the SALTOL QTL into Q5DB, the Elite Variety of Vietnam Using Marker- Assisted - Selection (MAS). Am. J. BioScience 2013, 1(4), 80-84. doi: 10.11648/j.ajbio.20130104.15

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AMA Style

Luu Thi Ngoc Huyen, Luu Minh Cuc, Le Huy Ham, Tran Dang Khanh. Introgression the SALTOL QTL into Q5DB, the Elite Variety of Vietnam Using Marker- Assisted - Selection (MAS). Am J BioScience. 2013;1(4):80-84. doi: 10.11648/j.ajbio.20130104.15

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@article{10.11648/j.ajbio.20130104.15,
  author = {Luu Thi Ngoc Huyen and Luu Minh Cuc and Le Huy Ham and Tran Dang Khanh},
  title = {Introgression the SALTOL QTL into Q5DB, the Elite Variety of Vietnam Using Marker- Assisted - Selection (MAS)},
  journal = {American Journal of BioScience},
  volume = {1},
  number = {4},
  pages = {80-84},
  doi = {10.11648/j.ajbio.20130104.15},
  url = {https://doi.org/10.11648/j.ajbio.20130104.15},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajbio.20130104.15},
  abstract = {Rice is a main food crop in Vietnam and plays a key role in economic growth in this country. However, its production and cultivating areas are severely affected from the threats of devastation caused by the rise of sea level. In this study, we have focused on developing new rice lines with salinity tolerance and high yield by applying markers assisted selection (MAS). Total of 21 primers in the Saltol QTL region were checked with the two parents varieties to identify polymorphic primers for screening the Saltol QTL region of the breeding populations. The individual plants in BC1, BC2, and BC3 generations of the Q5DB/FL478 were analyzed to evaluate the introgression of Saltol fragment into Q5DB cultivar. After screening of 3 BC generations, the best individual plants of BC3F1 of the plant numbers QF-3-1, QF-3-2, QF4-3-3, QF6-3 with almost the recipient alleles were selected. Conventional breeding was developed on BC3F2 for selection of the new salt tolerance rice lines with high yield, resistance to the biotic stress. The salinity tolerance of the new lines was also performed using the standard system of screening.},
 year = {2013}
}

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TY  - JOUR
T1  - Introgression the SALTOL QTL into Q5DB, the Elite Variety of Vietnam Using Marker- Assisted - Selection (MAS)
AU  - Luu Thi Ngoc Huyen
AU  - Luu Minh Cuc
AU  - Le Huy Ham
AU  - Tran Dang Khanh
Y1  - 2013/11/30
PY  - 2013
N1  - https://doi.org/10.11648/j.ajbio.20130104.15
DO  - 10.11648/j.ajbio.20130104.15
T2  - American Journal of BioScience
JF  - American Journal of BioScience
JO  - American Journal of BioScience
SP  - 80
EP  - 84
PB  - Science Publishing Group
SN  - 2330-0167
UR  - https://doi.org/10.11648/j.ajbio.20130104.15
AB  - Rice is a main food crop in Vietnam and plays a key role in economic growth in this country. However, its production and cultivating areas are severely affected from the threats of devastation caused by the rise of sea level. In this study, we have focused on developing new rice lines with salinity tolerance and high yield by applying markers assisted selection (MAS). Total of 21 primers in the Saltol QTL region were checked with the two parents varieties to identify polymorphic primers for screening the Saltol QTL region of the breeding populations. The individual plants in BC1, BC2, and BC3 generations of the Q5DB/FL478 were analyzed to evaluate the introgression of Saltol fragment into Q5DB cultivar. After screening of 3 BC generations, the best individual plants of BC3F1 of the plant numbers QF-3-1, QF-3-2, QF4-3-3, QF6-3 with almost the recipient alleles were selected. Conventional breeding was developed on BC3F2 for selection of the new salt tolerance rice lines with high yield, resistance to the biotic stress. The salinity tolerance of the new lines was also performed using the standard system of screening.
VL  - 1
IS  - 4
ER  -

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