A1180V of Cardiac Sodium Channel Gene (SCN5A): Is It a Risk Factor for Dilated Cardiomyopathy or Just a Common Variant in Han Chinese?

Shen, Cheng; Xu, Lei; Yang, Zhiyin; Zou, Yunzeng; Hu, Kai; Fan, Zheng; Ge, Junbo; Sun, Aijun

doi:https://doi.org/10.1155/2013/659528

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Research Article | Open Access

Volume 35 | Article ID 659528 | https://doi.org/10.1155/2013/659528

A1180V of Cardiac Sodium Channel Gene (SCN5A): Is It a Risk Factor for Dilated Cardiomyopathy or Just a Common Variant in Han Chinese?

Cheng Shen,^1,2Lei Xu,¹Zhiyin Yang,³Yunzeng Zou,^1,4Kai Hu,¹Zheng Fan,⁵Junbo Ge,^1,4and Aijun Sun^1,4

Academic Editor: Stamatios Theocharis

Received17 Jun 2013

Accepted18 Sept 2013

Published20 Oct 2013

Abstract

Our previous study of a Chinese family with dilated cardiomyopathy (DCM) suggested that A1180V of the cardiac sodium channel gene (SCN5A) was associated with the disease within this family. According to data deposited in dbSNP, however, A1180V has been found in some small samples of the Asian population. In this study, we followed up the affected pedigree and expanded the investigation of the prevalence of A1180V in 460 unrelated healthy Han Chinese. Besides, we searched and analyzed it in other database as well. During the follow-up period, 1 A1180V carrier’s condition deteriorated alot, and another 4 carriers progressed to DCM or atrioventricular block (AVB). We also found that the A1180V was absent among the 460 individuals (0%, 0/460), and the carrier frequency of A1180V among Chinese was about 0.51% obtained from the 1000 genome project. In conclusion, our finding suggests that A1180V is a potential risk factor for DCM, and it is extremely rare among Healthy Han Chinese.

1. Introduction

Dilated cardiomyopathy (DCM) is the most common form of primary cardiomyopathy and is a leading cause for heart transplantations [1] approximately 30%–50% of affected individuals have a familial form of DCM [2]. To date, at least 40 disease genes have been reported to be associated with familial DCM [3], in which; mutations of SCN5A gene which encodes the alpha subunit of the major sodium channel in the heart have been demonstrated to result in myocardial damage and DCM [4, 5]. Previously, SCN5A has been implicated in conduction disturbances and several electrophysiological phenotypes [6, 7], including atrioventricular block (AVB), long QT syndrome, sick sinus syndrome, sudden infant death syndrome, and atrial fibrillation [8, 9]. Meanwhile, more and more SCN5A mutations were discovered to be related with DCM, such as A1180V [10], D1275N [11], W156X, R225W [12], and R222Q [13].

We first reported finding A1180V, a single nucleotide mutation, , that occurred in exon 20 of the SCN5A gene in a three-generation Chinese family with age-related DCM and progressive AVB in 2008 [10]. In that study, A1180V was detected in all studied patients and in six younger unaffected members of the pedigree. The mutation, however, was not found in 200 healthy Han Chinese controls. Experimental data revealed that A1180V channels exhibited a negative transform of voltage-dependent inactivation of the cardiac sodium channels, which lead to a rate-dependent sodium current reduction, and a moderate increase in late sodium current. Correspondingly, our clinical study revealed QRS widening at high heart rates and QTc prolongation at rest in unaffected carriers. These findings suggest that A1180V is a functional mutation that can contribute to the development of DCM [10].

However, data deposited in the dbSNP database [14] show that A1180V has been found in two small sample groups of the Asian population. In the first sample group, A1180V (dbSNP refSNP number, rs41310765) was present in a cohort of 47 Han people (R31_HANLA) who were part of the Los Angeles Panel at a carrier frequency of 6.4%. In another sample group (HER_ASIAN-PANEL) of 16 individual Asians selected from the Coriell Cell Repository, genetic sequencing yielded a carrier frequency of 6.2% (http://www.ncbi.nlm.nih.gov/projects/SNP/snp_ref.cgi?rs=41310765). To date, the data obtained from the dbSNP database show that A1180V is virtually absent in other ethnic groups. Thus, these unpublished data suggest that A1180V might be a common variant in Asians, which is inconsistent with our previous observations.

In order to further evaluate the pathogenic role of A1180V in familial DCM and make sure the frequency of A1180V among Healthy Han Chinese, we followed up the three-generation Chinese family and expanded the control size to screen for A1180V. Besides, we analyzed its frequency in other database as well.

2. Materials and Methods

2.1. Study Population

We followed up all the A1180V carriers and noncarriers of the three-generation Chinese family for 6 years. All individuals were interviewed with the symptoms of cardiovascular disease, such as typical chest pain, palpitation, cyanosis, and syncope. 12-lead electrocardiogram (ECG) and echocardiography were performed on the family members to make a diagnosis.

460 unrelated Healthy Han Chinese living in Shanghai, ranging from 20 to 70 years old, were enrolled to the control group from a National High Technology Research and Development Program (the National 863 Program) of China. Individuals with known cardiovascular risk factors other than age, gender, and smoking history were excluded. The enrolled participants did not show any abnormalities under comprehensive clinical evaluation, including review of medical history, physical examination, 12-lead ECG, submaximal exercise ECG, and blood biochemical examinations.

Each of the participants and individuals whose data are included here signed an informed consent form after a complete and clear explanation of the nature and purpose of the study. The study was approved by the local Ethics Committee of Zhongshan Hospital, Fudan University.

2.2. Genotype Determination

DNA was extracted from 2 mL venous blood according to kit procedure (Sigma, USA) and stored at −80°C. The exon 20 of SCN5A was amplified by polymerase chain reaction (PCR). A 20 uL mixture was prepared for each reaction and included 1x HotStarTaq buffer, 2.0 mM Mg²⁺, 0.2 mM dNTP, 0.2 μM of each primer, 1 U HotStarTaq polymerase (Qiagen Inc.) and 1 μL template DNA. The DNA was amplified by cycling at 95°C for 15 min; 11 cycles of 94°C for 15 s, 62°C–0.5°C per cycle for 40 s, 72°C for 1 mins; 24 cycles of 94°C for 15 s, 57°C for 30 s, 72°C for 1 mins; 72°C for 2 min. The oligonucleotide sequences of the primers were as follows: 5′-ACCCCCATCATCGTAGCTCTT-3′ and 5′-TCCTGCTCTGGCCTCCATAC-3′. The PCR products were purified by 1 U SAP and 6 U Exo I, and they were added into 8 μL PCR products. The mixture was incubated at 37°C for 60 mins, followed by incubation at 70°C for 10 mins. Reaction mixture included 2 μL BigDye3.1 mix, 2 μL sequencing primer (0.4 μM, 5′-ACCCCCATCATCGTAGCTCTT3′) and 1-2 μL purified PCR product. The cycling program was 96°C for 1 min; 28 cycles of 96°C for 10 s, 50°C for 5 s, 60°C for 4 mins. The sequencing products were analyzed by an ABI3730XL DNA Analyzer. The results were analyzed by the Polyphred software and revised manually.

2.3. Database Retrieval

We searched A1180V of SCN5A in 1000 genomes project [15]. Besides, we have excluded some familial data to make sure that the frequency of A1180V from 1000 genomes project was calculated from unrelated individuals.

2.4. Statistical Analyses

All statistical analyses were performed with the SAS statistical program. Comparison of the A1180V carrier frequency between our studies and the results of dbSNP database was tested by using the Fisher’s exact test. Statistical significance was determined at .

3. Results

Combined with our former study and the 6 years of clinical followup of the affected pedigree, we discovered that A1180V related DCM was preceded by progressive AVB which mostly occurred in the third decade of life. During the follow-up period, the condition of an A1180V carrier (II-8 in Figure 1) who had been diagnosed with DCM before aggravated gradually and she now was diagnosed with severe congestive heart failure. Besides, III-1 with A1180V, developed to DCM and complete atrioventricular block (AVB) and another 3 carriers (III-2, III-3, III-13) progressed to different degree of AVB. Figure 2 displays changes of echocardiograms of III-1 and ECG recordings of III-2 in the follow-up period. None of the individuals in the pedigree showed any sign of long-QT or Brugada syndrome and other diseases.

Figure 1

Pedigree of the affected family. This figure mostly indicates the results of the 6 years followup, and the former characteristics of this family could be obtained from our published data in 2008 [10]. Individuals of the affected family are represented by squares (males) and circles (females). Five carriers whose disease have progressed to severe DCM or AVB during the follow-up period are marked by darkened symbols. The slash indicates the decreased people, and the arrow represents the proband. A1180V carriers and noncarriers are represented by “+” and “−”, respectively.

(a)

(b)

(c)

(d)

After screening A1180V in 460 individuals, we found that A1180V was absent in this group (0/460, 0%). Figure 3(a) gives an example of the sequencing results, and Figure 3(b) is a sequencing result of a A1180V carrier in 2007. The combined carrier frequency, 0% (i.e., 0 out of 660 cases), of A1180V from our studies remarkably suggests that A1180V is not a common variant among Healthy Chinese, and the data significantly differs from that in the dbSNP database .

(a)

(b)

Data from the 1000 genomes project revealed a carrier frequency of 0.51% (i.e., 1 out of 197 cases) for A1180V among Chinese participants. The data was obtained by analyzing the sequencing results of 97 northern and 100 southern Chinese adults. Since the southern Chinese group was familial related, the data from known offspring were excluded from the calculation of the carrier frequency to make sure the value was calculated from unrelated individuals (http://browser.1000genomes.org/Homo_sapiens/Variation/Population?db=core;r=3:38616415-38617415;v=rs41310765;vdb=variation;vf=12532571). The data also dramatically differs from those deposited in the dbSNP database . Table 1 summarizes the results of different studies of the carrier frequency of A1180V.

4. Discussion

We have presented the followup and sequencing data identifying that A1180V of SCN5A was a potential risk factor for DCM, and it is absent among healthy Han Chinese. Compared with the former results in 2008, 5 A1180V carriers exhibited age-related progressive AVB and DCM, and the A1180V carrier frequency among Han Chinese in our study is 0% out of 660 control cases.

Evidence is now being accumulated that SCN5A mutations are associated with development of cardiac dilation [13, 16–18]. McNair et al. have reported that mutations in SCN5A were detected in 1.7% of DCM families with a shared mechanism of disruption of the voltage-sensing mechanism of this channel leading to DCM [19]. Moreover, some SCN5A mutations which have been found to be a primary factor of leading DCM were characterized by an age-related phenotype of cardiac dilation with different types of arrhythmia [11, 20]. And the pathogenic characteristic of A1180V conformed to the pattern as well. Since the cardiac sodium channel is principally in charge of the fast depolarization of the myocardium [21], some suggested that the cardiac dilation may result from long-term arrhythmia [22, 23]. However, our experimental evidence indicated that A1180V could alter calcium homeostasis as a consequence of alterations in intracellular sodium concentrations, and the former condition might damage myocardium directly and subsequently lead to cardiac dilation [10].

Our data have revealed that A1180V specifically appeared in the affected pedigree and was absent among healthy controls, however, why do such significant discrepancies exist for A1180V among different studies of the same ethnic population? Possible explanations for the discrepancies include the following. (1) Different Asian ethnic subgroups may have different genetic backgrounds that cause differing prevalence of a genetic variant. For example, R1193Q (another SCN5A variant) is more prevalent in Asians living in Taiwan (frequency~12%) compared to Asians living in Japan (~2%) [24]. Most Chinese-Americans are descendants of people from southern China, so they may have a genetic background that differs slightly from the majority of the subjects enrolled in our studies. The population of the Shanghai area consists mostly of people who have immigrated from other areas of China. (2) Relatively small sample sizes may also account for high carrier frequency of those data deposited in the dbSNP database. (3) The sequencing depth of different sequencing technologies varies greatly within and between experiments, and it may introduce bias as well [25].

At present, lots of gene mutations were found playing pathogenic roles in both familial and sporadic diseases [26, 27], then another question is that whether A1180V would be a risk factor among sporadic DCM patients as well. In our recent studies of the distributions of candidate mutations in DCM-related genes in sporadic DCM patients conducted using high-throughput sequencing technology, we identified A1180V in 1 out of 68 patients with DCM. The patient, a 41-year-old male, was diagnosed as having DCM with NYHA class I, LVEDD 78 mm, and LVEF 24%, but no other diseases. However, the patient carrying A1180V also carries other mutations in several DCM-related genes, including G1117S, P1119R, Y498H of LAMA4, W768S of RBM20, P398S of VCL, and R659W of SCN5A. The last two mutations have never been reported previously. Although we found that A1180V might also be a risk factor in sporadic DCM patients, we cannot exclude the possibility that A1180V could work together with other gene mutations.

In addition to genetic factors, our previous study of the DCM pedigree carrying A1180V demonstrated that the A1180V channel lost current only at high heart rates, which suggested that physical activity and lifestyle that increased average daily heart rate might enhance the phenotype for A1180V carriers [10]. Therefore, environmental factors may also play a role in expression of the pathogenic mechanism of A1180V. Further studies of genetic and environmental factors and followup of A1180V carriers will provide more information about the relationship between the mutation and the risk of DCM. Studies of transgenic mice may also provide a step to test the hypothesis and to explore the underlying mechanism.

5. Conclusions

In conclusion, herein we provide evidence that the A1180V is a potential cause for DCM rather than a common variant in Han Chinese, although additional genetic or environmental factors may potentiate the risk for DCM in A1180V carriers.

Conflict of Interests

The authors declare that there is no conflict of interests regarding the publication of this paper.

Author’s Contribution

Cheng Shen and Lei Xu contributed equally to this work.

Acknowledgments

The authors appreciate the individuals and clinicians who have submitted clinical samples or offered medicine help. Also thank for the sequencing services from the Center of Genetic and Genomic Analysis, Genesky Biotechnologies Inc., Shanghai. This study is supported by the Grant (81170202) to Aijun Sun from the Surface Project of the National Natural Science Foundation of China (NSFC) and the Grant (09SG03) to Aijun Sun from the Dawn Plan of Shanghai Education Committee.

References

B. J. Maron, J. A. Towbin, G. Thiene et al., “Contemporary definitions and classification of the cardiomyopathies: an American Heart Association Scientific Statement from the Council on Clinical Cardiology, Heart Failure and Transplantation Committee; Quality of Care and Outcomes Research and Functional Genomics and Translational Biology Interdisciplinary Working Groups; and Council on Epidemiology and Prevention,” Circulation, vol. 113, no. 14, pp. 1807–1816, 2006.
View at: Publisher Site | Google Scholar
R. Roncarati, C. V. Anselmi, P. Krawitz et al., “Doubly heterozygous LMNA and TTN mutations revealed by exome sequencing in a severe form of dilated cardiomyopathy,” European Journal of Human Genetics, vol. 21, no. 10, pp. 1105–11011, 2013.
View at: Publisher Site | Google Scholar
D. Fatkin, R. Otway, and Z. Richmond, “Genetics of dilated cardiomyopathy,” Heart Failure Clinics, vol. 6, no. 2, pp. 129–140, 2010.
View at: Publisher Site | Google Scholar
E. Schulze-Bahr, “Cardiac sodium channels: dysregulation meets myocardial failure,” Cardiovascular Research, vol. 75, no. 3, pp. 455–456, 2007.
View at: Publisher Site | Google Scholar
T. M. Olson, V. V. Michels, J. D. Ballew et al., “Sodium channel mutations and susceptibility to heart failure and atrial fibrillation,” Journal of the American Medical Association, vol. 293, no. 4, pp. 447–454, 2005.
View at: Publisher Site | Google Scholar
A. A. M. Wilde and C. R. Bezzina, “Genetics of cardiac arrhythmias,” Heart, vol. 91, no. 10, pp. 1352–1358, 2005.
View at: Publisher Site | Google Scholar
A. L. George Jr., “Inherited disorders of voltage-gated sodium channels,” Journal of Clinical Investigation, vol. 115, no. 8, pp. 1990–1999, 2005.
View at: Publisher Site | Google Scholar
A. A. M. Wilde and R. Brugada, “Phenotypical manifestations of mutations in the genes encoding subunits of the cardiac sodium channel,” Circulation Research, vol. 108, no. 7, pp. 884–897, 2011.
View at: Publisher Site | Google Scholar
D. Darbar, P. J. Kannankeril, B. S. Donahue et al., “Cardiac sodium channel (SCN5A) variants associated with atrial fibrillation,” Circulation, vol. 117, no. 15, pp. 1927–1935, 2008.
View at: Publisher Site | Google Scholar
J. Ge, A. Sun, V. Paajanen et al., “Molecular and clinical characterization of a novel SCN5A mutation associated with atrioventricular block and dilated cardiomyopathy,” Circulation, vol. 1, no. 2, pp. 83–92, 2008.
View at: Publisher Site | Google Scholar
W. P. McNair, L. Ku, M. R. G. Taylor et al., “SCN5A mutation associated with dilated cardiomyopathy, conduction disorder, and arrhythmia,” Circulation, vol. 110, no. 15, pp. 2163–2167, 2004.
View at: Publisher Site | Google Scholar
C. R. Bezzina, M. B. Rook, W. A. Groenewegen et al., “Compound heterozygosity for mutations (W156X and R225W) in SCN5A associated with severe cardiac conduction disturbances and degenerative changes in the conduction system,” Circulation Research, vol. 92, no. 2, pp. 159–168, 2003.
View at: Google Scholar
S. A. Mann, M. L. Castro, M. Ohanian et al. et al., “R222Q SCN5A mutation is associated with reversible ventricular ectopy and dilated cardiomyopathy,” American College of Cardiology, vol. 60, no. 16, pp. 1566–1573, 2012.
View at: Publisher Site | Google Scholar
S. T. Sherry, M. H. Ward, M. Kholodov et al., “dbSNP: the NCBI database of genetic variation,” Nucleic Acids Research, vol. 29, no. 1, pp. 308–311, 2001.
View at: Google Scholar
G. R. Abecasis, D. Altshuler, A. Auton et al. et al., “A map of human genome variation from population-scale sequencing,” Nature, vol. 467, no. 7319, pp. 1061–1073, 2010.
View at: Publisher Site | Google Scholar
K. Y. van Spaendonck-Zwarts, I. A. van Rijsingen, M. P. van den Berg et al., “Genetic analysis in 418 index patients with idiopathic dilated cardiomyopathy: overview of 10 years' experience,” European Journal of Heart Failure, vol. 15, no. 6, pp. 628–636, 2013.
View at: Publisher Site | Google Scholar
P. Gosselin-Badaroudine, I. D. Keller, I. D. Hai Huang et al., “A proton leak current through the cardiac sodium channel is linked to mixed arrhythmia and the dilated cardiomyopathy phenotype,” PLoS One, vol. 7, no. 5, Article ID e38331, 2012.
View at: Google Scholar
E. Rampersaud, J. D. Siegfried, N. Norton, D. Li, E. Martin, and R. E. Hershberger, “Rare variant mutations identified in pediatric patients with dilated cardiomyopathy,” Progress in Pediatric Cardiology, vol. 31, no. 1, pp. 39–47, 2011.
View at: Publisher Site | Google Scholar
W. P. McNair, G. Sinagra, M. R. Taylor et al., “SCN5A mutations associate with arrhythmic dilated cardiomyopathy and commonly localize to the voltage-sensing mechanism,” American College of Cardiology, vol. 57, no. 21, pp. 2160–2168, 2011.
View at: Publisher Site | Google Scholar
R. Shi, Y. Zhang, C. Yang et al., “The cardiac sodium channel mutation delQKP, 1507-1509 is associated with the expanding phenotypic spectrum of LQT3, conduction disorder, dilated cardiomyopathy, and high incidence of youth sudden death,” Europace, vol. 10, no. 11, pp. 1329–1335, 2008.
View at: Publisher Site | Google Scholar
Y. Ruan, N. Liu, and S. G. Priori, “Sodium channel mutations and arrhythmias,” Nature reviews. Cardiology, vol. 6, no. 5, pp. 337–348, 2009.
View at: Publisher Site | Google Scholar
W. A. Groenewegen and A. A. Wilde, “Letter regarding article by McNair et al, ‘SCN5A mutation associated with dilated cardiomyopathy, conduction disorder, and arrhythmia’,” Circulation, vol. 112, no. 1, pp. e9–e10, 2005.
View at: Google Scholar
C. R. Bezzina and C. A. Remme, “Dilated cardiomyopathy due to sodium channel dysfunction: what is the connection?” Circulation, vol. 1, no. 2, pp. 80–82, 2008.
View at: Publisher Site | Google Scholar
H. W. Hwang, J. J. Chen, Y. J. Lin et al., “R1193Q of SCN5A, a Brugada and long QT mutation, is a common polymorphism in Han Chinese,” Journal of Medical Genetics, vol. 42, no. 2, article e7, 2005.
View at: Google Scholar
C. Garner, “Confounded by sequencing depth in association studies of rare alleles,” Genetic Epidemiology, vol. 35, no. 4, pp. 261–268, 2011.
View at: Publisher Site | Google Scholar
N. Norton, D. Li, E. Rampersaud et al., “Exome sequencing and genome-wide linkage analysis in 17 families illustrate the complex contribution of TTN truncating variants to dilated cardiomyopathy,” Circulation, vol. 6, no. 2, pp. 144–153, 2013.
View at: Publisher Site | Google Scholar
H. Kabata, T. Satoh, M. Kataoka et al., “BMPR2 mutations, clinical phenotypes and outcomes of Japanese patients with sporadic or familial pulmonary hypertension,” Respirology, vol. 18, no. 7, pp. 1076–1082, 2013.
View at: Publisher Site | Google Scholar

Copyright

Copyright © 2013 Cheng Shen et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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