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Abstract
Background Three hours per week of vigorous physical activity is found to be associated with reduced odds of sleep-disordered breathing.
Aim To investigate whether 12 weeks of high-intensity interval training (HIIT) reduced the apnoea–hypopnea index (AHI) in obese subjects with moderate-to-severe obstructive sleep apnoea.
Methods In a prospective randomised controlled exercise study, 30 (body mass index 37±6 kg/m2, age 51±9 years) patients with sleep apnoea (AHI 41.5±25.3 events/hour) were randomised 1:1 to control or 12 weeks of supervised HIIT (4×4 min of treadmill running or walking at 90%–95% of maximal heart rate two times per week).
Results In the HIIT group, the AHI was reduced by 7.5±11.6 events/hour (within-group p<0.05), self-reported sleepiness (Epworth scale) improved from 10.0±3.6 to 7.3±3.7 (between-group p<0.05) and maximal oxygen uptake improved from 28.2±7.4 to 30.2±7.7 mL/kg/min (between-group p<0.05) from baseline to 12 weeks. The AHI, self-reported sleepiness and VO2maxwere unchanged from baseline to 12 weeks in controls (baseline AHI 50.3±25.5 events/hour, Epworth score 5.9±4.3, maximal oxygen uptake 27.0±6.8 mL/kg/min). Body weight remained unchanged in both groups.
Conclusion Twelve weeks of HIIT improved the AHI and self-reported daytime sleepiness in subjects with obese sleep apnoea without any change in the desaturation index and body weight.
- OSA
- HIIT
- daytime sleepiness
- CVD risk
- endurance exercise
- VO2max
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Key messages
High-intensity exercise training improves:
The apnoea–hypopnea index
Self-reported sleepiness
Maximal oxygen uptake.
What are the new findings?
High-intensity exercise training improves the apnoea–hypopnea index.
High-intensity exercise training improves daytime sleepiness.
High-intensity exercise training is feasible in obese subjects with obstructive sleep apnoea.
How might it impact on clinical practice in the near future?
High-intensity exercise training could be a time- efficient exercise alternative for subjects diagnosed with obstructive sleep apnoea and high cardiovascular comorbid clustering.
Introduction
Obstruction of breathing during sleep due to upper airway collapse, also known as obstructive sleep apnoea (OSA),1 is associated with physical inactivity2 3 and several inactivity-related comorbidities such as obesity, hypertension, type 2 diabetes and cardiovascular disease.4 Sleep deprivation and daytime sleepiness are associated with low levels of physical activity5 and could induce a vicious cycle of physical inactivity and low exercise motivation,6 insomnia and comorbidity clustering.
In a cohort study, questionnaire-based, self-reported vigorous physical activity three times per week or more was found to be associated with reduced odds of developing sleep-disordered breathing.7 High-intensity interval training (HIIT) is found superior to moderate exercise in improving cardiovascular disease-related comorbidities and maximal oxygen consumption (VO2max) in several cardiometabolic high-risk populations.8 9 As OSA is associated with obesity and several high-risk cardiovascular disease comorbidities, HIIT as a possible treatment of both the obstructive sleep disorder and cardiovascular risk factors should be studied.
Standard treatment with continuous positive airway pressure (CPAP) and reduction in body weight improves airflow obstruction (measured as the apnoea–hypopnea index (AHI)), daytime sleepiness and several OSA-related comorbidities.5 However, long-term adherence to both treatments is moderate,5 10 and focus on other positive lifestyle changes is limited. Modest improvements in AHI, self-reported sleepiness, VO2max and quality of life are reported in exercise interventions with moderate intensity,11 12 in combination with breathing exercises,11 diet,13 CPAP14 and strength exercise.12 In a meta-analysis, AHI decreased with 6.7 events/hour (32% reduction) after exercise, independent of change in body weight.15
To our knowledge, no study has investigated the effect of HIIT on AHI in subjects diagnosed with OSA. We hypothesised that 12 weeks of two weekly sessions of HIIT would significantly improve AHI in obese patients diagnosed with moderate-to-severe OSA.
Materials and methods
Thirty subjects with moderate-to-severe OSA were recruited from the Department of Thoracic Medicine at St. Olav’s Hospital, Trondheim University Hospital, or through public announcements. After initial screening, patients were randomised 1:1 into HIIT or a control group (CON) through a study-specific web randomisation database at the Unit of Applied Clinical Research, Norwegian University of Science and Technology (webcrf.medisin.ntnu.no). No stratification was used. The inclusion criteria were a body mass index (BMI) ≥30 kg/m2 and an AHI ≥15. Exclusion criteria were inability to exercise due to musculoskeletal conditions and known ischemic cardiovascular disease. Figure 1 describes the patient flow in the study.
Study flow chart. CON, control group; HIIT, high-intensity interval training.
Ethics
The study was approved by the Regional Medical Research Ethic Committee (no 2010/1539), registered in the clinical trials database (NCT01215617) and performed according to the Declaration of Helsinki. All subjects signed written informed consent before randomisation in the study.
Protocol
After the initial screening investigation (baseline) and randomisation, subjects in the HIIT group performed 12 weeks of supervised HIIT, two times per week, while the CON group was encouraged to continue their normal lifestyle. All subjects continued their normal CPAP use. At 12 weeks, postintervention testing of primary and secondary outcomes was performed. The primary study outcome was AHI measured through respiratory polygraphy registrations. Secondary outcomes included: Epworth sleepiness questionnaire (Norwegian version),16 VO2max, flow–volume spirometry and blood biomarkers.
Exercise training intervention
HIIT was performed as 4×4 min of treadmill walking or running at 90%–95% of maximal heart rate (HRmax), two times per week, in the department’s training facility at St. Olav’s Hospital, Trondheim University Hospital.8 Each training session started with 10 min of warm-up period at ∼70% of HRmax. Between every interval of 4 min and after the last interval, the subjects walked at ∼70% of HRmax for 3 mins. The subjects used heart rate (HR) monitors and their target HR to control exercise intensity and aimed to reach their individual target HR zone after 1–1.5 min of exercise during each interval. The exercise physiologist supervising all training sessions checked the target HR of the subjects. Treadmill speed or grade was increased throughout the training period to maintain the target exercise intensity.
Sleep evaluation
Subjects underwent one full night of respiratory polygraphy registrations with ambulatory equipment (Embletta; Embla, Ontario, Canada) at home at baseline and 12 weeks. A research nurse fitted the equipment to the subjects, and patients slept without using CPAP during the nights of the registration. The subjects reported sleeping patterns to the nurse the morning after the registration and answered the Epworth sleepiness questionnaire. Blinded polygraphy analysis was performed by a specialist in clinical neurophysiology using Somnologica studio 5.1 (Micromed Mogliano Veneto, Triveso, Italy). To compensate for the lack of electroencephalography during the registration, limiting scores of hypopneas connected to arousals, respiration was scored according to the slightly modified ‘Chicago criteria’ (without arousals),17 which has a higher sensitivity than the American Academy of Sleep Medicine (AASM) 2007 criteria (comparable to AASM 2012).18–20 A drop in oxygen saturation of 4% or more was defined as a desaturation event.
Cardiopulmonary exercise testing
To evaluate the effect of the training intervention and to identify the target exercise intensity, a VO2max treadmill running/walking test was performed using indirect breath-by-breath calorimetry (MMX-II; CORTEX Biophysik, Leipzig, Germany). After a 15 min warm-up at ∼70% of HRmax, the test equipment was fitted to the subjects. After 5 mins of submaximal walking, a gradual increase in workload was made until the patients reached exhaustion. Using an individualised protocol,21 speed for running (1 km/hour) or grade for walking (2%) was increased approximately every minute, while grade (5% for running) and speed (4–6.5 km/hour for walking) were maintained. VO2max was defined as the mean of the highest three consecutive 10 s measurements where the VO2 leveled off despite an increase in intensity and with a respiratory quotient value above 1.10. HR was recorded continuously, and lactate was measured from a capillary blood sample immediately after the test. HR recovery was calculated as HRmax minus HR in 1 min after the end of exercise. Maximal voluntary ventilation (MVV) was estimated according to Wassermann to 40xFEV1.22
Clinical measurements
Body weight and body composition were measured using a bioimpedance scale (Omrun BF500; Omron Healthcare, Kyoto, Japan). Resting HR and systolic and diastolic blood pressure were measured with a polygraph (Tango+; SunTech Medical, Morrisville, North Carolina, USA). Venous plasma and serum samples were collected after overnight fasting for standard haematology and blood biomarker analysis at the Department of Clinical Chemistry, St. Olav’s Hospital, Trondheim University Hospital.
Lung function tests
A research nurse performed spirometry and gas diffusion tests using the Sensormedics Vmax22 Encore (CareFusion, San Diego, California, USA) in accordance with the American Thoracic Society/European Respiratory Society criteria. The spirometer was calibrated daily. The reported value represents the better of at least two acceptable spirometry trials with a variation of less than 150 mL for forced expiratory volume at 1 s and forced vital capacity. Diffusing capacity of the lung for carbon monoxide (DLCO) was measured by the single-breath method with a gas mixture containing 0.3% carbon monoxide and 0.3% methane. The procedure was repeated with at least 4 min between each trial up to a maximum of four trials, until two tests of acceptable quality showed DLCO within 3 mL/min/mm Hg or within 10% of the highest value, and the mean of these values were recorded.
Statistics
IBM SPSS version 21 was used for statistical analysis (IBM SPSS Statistics; IBM, New York, USA). The one-way analysis of variance and the repeated-measure analysis of covariance (ANCOVA) were used to test statistical significance within or between groups. Baseline values were used as covariables in the ANCOVA analysis to account for any between-group differences at baseline. A two-sided p value ≤0.05 was used for statistical significance. The study sample size was based on expectations of an AHI reduction of 4±5 events/hour, 80% power and a two-sided p value of 5%, requiring 15 subjects in each group.
Results
Baseline characteristics
Table 1 describes the baseline characteristics. Despite using a randomisation database, by chance, AHI was significantly lower, and the Epworth score was significantly higher in the HIIT group compared with CON at baseline (p≤0.05). There were no significant differences between groups in the remaining variables at baseline. At 12 weeks, total body fat in the HIIT group (∼2%) and skeletal muscle mass in the CON group (∼1%) were significantly reduced from baseline (p≤0.05). There was no significant between-group changes in BMI. Leptin was significantly reduced from 1747±1419 to 1412±1047 pmol/L from baseline to 12 weeks in the HIIT group (p=0.015), with no statistical change in control (12-week leptin 1276±594 pmol/L (p=0.393)). There were no statistical changes in clinical biomarkers from blood, blood pressure, flow–volume spirometry or pulmonary diffusion capacity in the HIIT or CON group from baseline to 12 weeks (12-week data not shown).
Baseline characteristics
Sleep data
Twelve weeks of HIIT improved AHI by 24% (p≤0.05), with no change in the CON group. There was no between-group difference in AHI from the baseline to the 12-week follow-up (p=0.2) (figure 2A). The Epworth self-reported sleepiness scale was significantly improved in the HIIT group compared with the CON group (p≤0.05) at 12 weeks (figure 2B). There was no change in nightly oxygen saturation or number of desaturation events in the HIIT or CON groups from baseline to 12 weeks (figure 2C,D). None of the subjects reported change in CPAP use during the intervention.
Change in AHI (A), oxygen desaturation index (C) and oxygen saturation (D) measured by respiratory polygraphy and self-reported daytime sleepiness measured with the Epworth scale (B) from baseline to 12 weeks. *Significant within-group difference from baseline (p≤0.05); **Significant between-group difference after the intervention (p≤0.05). AHI, apnoea–hypopnea index; CON, control group; HIIT, high-intensity interval training.
Cardiopulmonary exercise testing
VO2max increased by 2.0 mL/kg/min in the HIIT group at 12-week testing and was significantly improved compared with the CON group (p≤0.05). The change in VO2max did not correlate with the change in AHI (r=−0.11). Maximal ventilation (VE) was increased in the HIIT group at 12 weeks (p≤0.05) (table 2). As the maximal VE increased at 12 weeks in the HIIT group, the breathing reserve was decreased and the VE/MVV ratio increased and was significantly different from the CON group at 12 weeks (p≤0.05) (table 2).
Cardiopulmonary exercise testing
Exercise intervention
Subjects in the HIIT group performed 21±3 supervised training sessions (88% compliance). The mean exercise HR was 151±16 beats/min (92% of HRmax). The exercise HR was within the target zone and unchanged throughout the 12 weeks of supervised exercise. Two subjects in the HIIT group dropped out of training after 8 and 11 training sessions—one due to lack of time and one due to lower back pain.
Discussion
The main finding in this study was that 12 weeks of HIIT significantly improved AHI within the group, while the desaturation index remained stable. The improvement in AHI was accompanied by a significant improvement in the self-reported Epworth sleepiness score in the HIIT group compared with the CON group. Improvement in obstructive breathing during sleep most likely improved sleep quality and, therefore, also reduced self-reported daytime sleepiness in the HIIT group, with no significant changes in the CON group. The 24% improvement in AHI was modest and comparable to changes seen after moderate exercise training.15
Several mechanisms for improvement in AHI with moderate exercise training have been discussed. There was no change in body weight after HIIT. Hence, weight reduction per se is not the underlying mechanism for AHI improvement.11 12 The HIIT group did display a small decrease in total body fat (2%), with no significant change in muscle mass. Reduced body fat in the pharyngeal/neck region could improve upper airway airflow.1 Increased physical activity could improve venous blood flow and reduce daytime leg oedema, resulting in reduced upper airway night-time oedema and improved upper airway airflow and AHI during sleep23; however, we cannot confirm these mechanisms in our study.
An interaction of leptin with sleep-disturbed breathing has been discussed.24 Leptin is highly associated with OSA and obesity,1 25 and CPAP treatment reduces leptin levels.26 We found a decrease in serum leptin after HIIT in our subjects, where the majority were CPAP users. The change in leptin did not correlate with the change in AHI (r=−0.21), and there were no group differences. Elevated leptin is associated with reduced ventilatory control and hypoventilation in obese patients with OSA,27 and leptin replacement reverses hypoventilation in a leptin-deficient obese-mice model28; hence, interaction between exercise, leptin and OSA should further be investigated.
Risk factors such as blood biomarkers and blood pressure were normal at baseline and did not change with exercise, indicating that our subjects were well medically managed at inclusion. HIIT increased VO2max by 7%. The increase in VO2max is similar to comparable fit populations performing HIIT29 30 but somewhat lower than studies of cardiovascular disease patients with lower baseline VO2max.8 The difference in improvement between studies could be due to the baseline VO2max level, the number of weekly sessions (only two sessions per week were used in the current study, while the majority of other studies have used three weekly sessions8 29 30) or the importance of training intensity, where exercising above 92% of HRmax was found to improve VO2max the most in patients with coronary artery disease.31 Low VO2max is one of the strongest prognostic factors for future cardiovascular disease and premature all-cause mortality,32 33 independent of obesity.34 35 As OSA is strongly associated with obesity and elevated cardiovascular disease risk, improving VO2max, transforming patients with OSA from ‘unfit and fat’ to ‘fit and fat’ is a feasible risk reduction strategy.36 We found no correlation between the change in VO2max and AHI in our study; therefore, the effect of HIIT on AHI is most likely unrelated to improved cardiopulmonary fitness measured as VO2max. None of the subjects were limited in their exercise performance by pulmonary diseases, as resting pulmonary function and diffusion capacity were normal at baseline and did not change at 12 weeks (table 1).11 To explain the effect of HIIT on AHI and sleep quality, other mechanisms (as discussed above) should be explored further.
Strengths and limitations
We had no electroencephalogram (EEG) data and could not score arousals during sleep. However, all registrations were interpreted according to the same criteria and blinded for intervention allocation. We scored respiratory polygraphy registrations according to ‘Chicago criteria’,17 which permit scoring of hypopneas without the accompanied desaturations or arousals (lack of EEG electrodes). It is uncertain if improved respiration during sleep would have been detected if conservative hypopnea criteria had been chosen. Criteria for scoring of respiration during sleep to diagnose OSA have been widely discussed.34 37 38 Using the most liberal criteria when comparing several registrations over time seems adequate in our study, both to compensate for the lack of EEG electrodes in registrations and to optimise the ability to detect any changes from preintervention to postintervention. The accompanied reduction of Epworth score indicates that the effect was clinically relevant.
The primary study parameter, AHI, was significantly higher in CON compared with HIIT at baseline despite the use of a randomisation database. In retrospect, stratification for AHI should have been done in the randomisation process. As the CON group did have significantly higher AHI than the HIIT group at baseline, one could argue that the decrease in AHI in the HIIT group is present despite a moderate baseline AHI and due to the intervention, and not due to high baseline values causing a larger statistical regression towards the mean with repeated measures, as seen in the CON group. To account for baseline group differences, ANCOVA analysis with baseline as a covariable was used for group comparison. The study did not control for the effect of training intensity, as it lacks a moderate-exercise training group. Therefore, we cannot conclude if the effect of training on OSA is dependent on exercise intensity.
Conclusion
Twelve weeks of HIIT two times per week significantly improves the AHI, self-reported daytime sleepiness and maximal oxygen uptake in obese patients with sleep apnoea without any change in body weight. The improvement in AHI was modest and independent of increase in VO2max, indicating that physical activity per se might be important for improvement in sleep quality in patients with sleep apnoea.
Perspectives
We have demonstrated that HIIT is a feasible treatment to improve AHI and reduced daytime sleepiness on top of standard CPAP treatment in subjects with moderate-to-severe OSA. To our knowledge, this is the first study on the effects of high-intensity exercise in subjects with OSA. Future studies should compare the effects of high-intensity and moderate-intensity exercises and different training volumes as data from the follow-up period indicates positive effects of moderate-intensity exercise as well. In addition, further investigations of the underlying mechanisms behind the positive effect of exercise training on sleep obstruction are warranted.
References
Footnotes
Acknowledgements We are highly grateful to the Unit of Applied Clinical Research, to the medical faculty at the Norwegian University of Science and Technology for supplying the webCRF randomisation database and to the research nurses Marit Tjelle Setvik and Anne Stine Fossum for their assistance with the data collection. In addition, we are highly grateful to our research subjects for devoting their time to participate in the study.
Contributors All authors meet the criteria for authorship defined by the International Committee of Medical Journal Editors.
Funding The SINTEF Unimed foundation partly funded the study.
Competing interests None declared.
Ethics approval The study was approved by the Regional Medical Research Ethic Committee (no 2010/1539), registered in the clinical trials database (NCT01215617) and performed according to the Declaration of Helsinki.
Provenance and peer review Not commissioned; externally peer reviewed.
Data sharing statement Unpublished data in the study will be prepared and submitted for peer review after the publication of the current paper.