Skip to main content
SpringerLink
Log in

Role of the Mechanosensitive Protein Piezo1 in Age-Dependent Changes in the Number of Fibroblasts and Blood Vessels in Human Skin

  • Published:
Advances in Gerontology Aims and scope Submit manuscript

Abstract

The goal of the work is to study the content of the mechanosensitive protein Piezo1 in fibroblasts and blood vessels of the human dermis from embryonic development to very old age (from 20 weeks of pregnancy to 85 years) and to define the value of Piezo1 for age-related changes in the number of fibroblasts and blood vessels in the human dermis. Piezo1, proliferating cell nuclear antigen (PCNA), and CD31, a marker of endothelial cells, were detected in skin sections by an indirect immunohistochemical technique. The results showed that the proportion of fibroblasts with positive staining for Piezo1 in human dermis decreases systematically from 20 weeks of pregnancy to 40 years old. From the age of 41, there is a sharp increase in the proportion of dermal fibroblasts with positive staining for Piezo1, with the maximum in the age group of 61–85 years. The Piezo1 content in the blood vessels of the human dermis decreases from 20 weeks of pregnancy to 40 years. A significant increase in the Piezo1 content in the blood vessels of the human dermis is then observed in the age group of 41–85 years. The age-related changes in the Piezo1 content in fibroblasts and blood vessels of the dermis are not associated with the age-related decrease in the total number and proportion of PCNA-positive fibroblasts or the number of blood vessels in the dermis.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Fig. 1.
Fig. 2.
Fig. 3.
Fig. 4.

Similar content being viewed by others

REFERENCES

  1. Gunin, A.G. and Golubtsova, N.N., Thyroid hormone receptors in human skin during aging, Adv. Gerontol., 2018, vol. 8, no. 3, pp. 216–223.

    Article  Google Scholar 

  2. Gunin, A.G., Petrov, V.V., Vasilieva, O.V., and Golubtsova, N.N., Age-related changes of blood vessels in the human dermis, Adv. Gerontol., 2015, vol. 5, no. 2, pp. 65–71.

    Article  Google Scholar 

  3. Albarran-Juarez, J., Iring, A., Wang, S., et al., Piezo1 and Gq/G11 promote endothelial inflammation depending on flow pattern and integrin activation, J. Exp. Med., 2018. pii jem.20180483.

  4. Borbiro, I. and Rohacs, T., Regulation of Piezo channels by cellular signaling pathways, Curr. Top. Membr., 2017, vol. 79, pp. 245–261.

    Article  CAS  Google Scholar 

  5. Cox, C.D., Bavi, N., and Martinac, B., Origin of the force: the force-from-lipids principle applied to Piezo channels, Curr. Top. Membr., 2017, vol. 79, pp. 59–96.

    Article  CAS  Google Scholar 

  6. Fritz, P., Hones, J., Lutz, D., et al., Quantitative immunohistochemistry: standardization and possible application in research and surgical pathology, Acta Histochem., 1989, vol. 37, suppl., pp. 213–219.

    CAS  Google Scholar 

  7. Gudipaty, S.A., Lindblom, J., Loftus, P.D., et al., Mechanical stretch triggers rapid epithelial cell division through Piezo1, Nature, 2017, vol. 543, no. 7643, pp. 118–121.

    Article  CAS  Google Scholar 

  8. Gunin, A.G., Kornilova, N.K., Vasilieva, O.V., and Petrov, V.V., Age-related changes in proliferation, the numbers of mast cells, eosinophils, CD45 positive cells in human dermis, J. Gerontol. A, 2011, vol. 66, pp. 385–392.

    Article  Google Scholar 

  9. Gunin, A.G., Petrov, V.V., Golubtzova, N.N., et al., Age-related changes in angiogenesis in human dermis, Exp. Gerontol., 2014, vol. 55, pp. 143–151.

    Article  CAS  Google Scholar 

  10. Li, X.F., Zhang, Z., Chen, Z.K., et al., Piezo1 protein induces the apoptosis of human osteoarthritis-derived chondrocytes by activating caspase-12, the signaling marker of ER stress, Int. J. Mol. Med., 2017, vol. 40, no. 3, pp. 845–853.

    Article  CAS  Google Scholar 

  11. Martinac, B. and Poole, K., Mechanically activated ion channels, Int. J. Biochem. Cell. Biol., 2018, vol. 97, pp. 104–107.

    Article  CAS  Google Scholar 

  12. Parpaite, T. and Coste, B., Piezo channels, Curr. Biol., 2017, vol. 27, pp. R250–R252.

    Article  CAS  Google Scholar 

  13. Qin, Z., Xia, W., Fisher, G.J., et al., YAP/TAZ regulates TGF-β/Smad3 signaling by induction of Smad7 via AP-1 in human skin dermal fibroblasts, Cell. Commun. Signaling, 2018, vol. 16, p. 18.

    Article  Google Scholar 

  14. Rizzardi, A.E., Johnson, A.T., Vogel, R.I., et al., Quantitative comparison of immunohistochemical staining measured by digital image analysis versus pathologist visual scoring, Diagn. Pathol., 2012, vol. 7, p. 42.

    Article  Google Scholar 

  15. Romac, J.M., Shahid, R.A., Swain, S.M., et al., Piezo1 is a mechanically activated ion channel and mediates pressure induced pancreatitis, Nat. Commun., 2018, vol. 9, no. 1, p. 1715.

    Article  Google Scholar 

  16. Saotome, K., Murthy, S.E., Kefauver, J.M., et al., Structure of the mechanically activated ion channel Piezo1, Nature, 2018, vol. 554, no. 7693, pp. 481–486.

    Article  CAS  Google Scholar 

  17. Seidal, T., Balaton, A.J., and Battifora, H., Interpretation and quantification of immunostains, Am. J. Surg. Pathol., 2001, vol. 25, pp. 1204–1207.

    Article  CAS  Google Scholar 

  18. Wang, A.S. and Dreesen, O., Biomarkers of cellular senescence and skin aging, Front. Genet., 2018, vol. 9, p. 247.

    Article  Google Scholar 

  19. Wei, W. and Ji, S., Cellular senescence: molecular mechanisms and pathogenicity, J. Cell. Physiol., 2018. https://doi.org/10.1002/jcp.26956

  20. Zhao, Q., Zhou, H., Chi, S., et al., Structure and mechanogating mechanism of the Piezo1 channel, Nature, 2018, vol. 554, no. 7693, pp. 487–492.

    Article  CAS  Google Scholar 

Download references

Funding

This work was supported by the Russian Foundation for Basic Research, project nos. 18-44-210001 and 19-415-210001, and the government of the Chuvash Republic.

Author information

Authors and Affiliations

  1. Chuvash State University, 428015, Cheboksary, Russia

    A. G. Gunin & N. N. Golubtzova

Authors
  1. A. G. Gunin
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. N. N. Golubtzova
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to A. G. Gunin.

Ethics declarations

Conflict of interests. The authors declare that they have no conflict of interest.

Statement of compliance with standards of research involving humans as subjects. The study was approved by the ethics committee of the medical faculty of Chuvash State University.

Additional information

Translated by I. Shipounova

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Gunin, A.G., Golubtzova, N.N. Role of the Mechanosensitive Protein Piezo1 in Age-Dependent Changes in the Number of Fibroblasts and Blood Vessels in Human Skin. Adv Gerontol 9, 411–416 (2019). https://doi.org/10.1134/S2079057019040088

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1134/S2079057019040088

Keywords:

Search

Navigation