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Multipotent mesenchymal stem cells of desquamated endometrium: Isolation, characterization, and application as a feeder layer for maintenance of human embryonic stem cells

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Abstract

In this study, we characterize new multipotent human mesenchymal stem cell lines (MSCs) derived from desquamated (shedding) endometrium of menstrual blood. The isolated endometrial MSC (eMSC) is an adhesive to plastic heterogeneous population composed mainly of endometrial glandular and stromal cells. The established cell lines meet the criteria of the International Society for Cellular Therapy for defining multipotent human MSCs of any origin. The eMSCs have positive expression of CD13, CD29, CD44, CD73, CD90, and CD105 markers and lack hematopoietic cell surface antigens CD19, CD34, CD45, CD117, CD130, and HLA-DR (class II). Multipotency of the established eMSCs is confirmed by their ability to differentiate into other mesodermal lineages, such as osteocytes and adipocytes. In addition, the isolated eMSCs partially (over 50%) express the pluripotency marker SSEA-4. However, they do not express Oct-4. Immunofluorescent analysis of the derived cells revealed the expression of the neural precursor markers nestin and β-III-tubulin. This suggests a neural predisposition of the established eMSCs. These cells are characterized by a high proliferation rate (doubling time 22–23 h) and a high colony-forming efficiency (about 60%). In vitro, the eMSCs undergo more than 45 population doublings without karyotypic abnormalities. We demonstrate that mitotically inactivated eMSCs are perfect feeder cells for maintenance of human embryonic stem cell lines (hESCs) C612 and C910. The eMSCs, being a feeder culture, sustain the hESC pluripotent status that verified by expression of Oct-4, alkaline phosphatase and SSEA-4 markers. The hESCs cocultured with the eMSCs retain their morphology and proliferative rate for more than 40 passages and exhibit the capability for spontaneous differentiation into embryoid bodies comprising three embryonic germ layers. Thus, an easy and noninvasive isolation of the eMSCs from menstrual blood, their multipotency and high proliferative activity in vitro without karyotypic abnormalities demonstrate the potential of use of these stem cells in regenerative medicine. Using the derived eMSCs as the feeder culture eliminates the risks associated with animal cells while transferring hESCs to clinical setting.

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Abbreviations

MSCs:

mesenchymal stem cells

eMSCs:

endometrial MSCs

MEFs:

mouse embryonic fibroblasts

hESCs:

human embryonic stem cells

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Authors and Affiliations

  1. Institute of Cytology, Russian Academy of Sciences, St. Petersburg, Russia

    V. I. Zemelko, T. M. Grinchuk, A. P. Domnina, I. V. Artzibasheva, V. V. Zenin & N. N. Nikolsky

  2. Faculty of Medical Physics, St. Petersburg State Polytechnical University, St. Petersburg, Russia

    N. N. Nikolsky

  3. International Center of Reproductive Medicine, St. Petersburg, Russia

    A. A. Kirsanov, N. K. Bichevaia & V. S. Korsak

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  1. V. I. Zemelko
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  2. T. M. Grinchuk
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  3. A. P. Domnina
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  4. I. V. Artzibasheva
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  5. V. V. Zenin
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  6. A. A. Kirsanov
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  7. N. K. Bichevaia
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  8. V. S. Korsak
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  9. N. N. Nikolsky
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Correspondence to V. I. Zemelko.

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Original Russian Text © V.I. Zemelko, T.M. Grinchuk, A.P. Domnina, I.V. Artzibasheva, V.V. Zenin, A.A. Kirsanov, N.K. Bichevaia, V.S. Korsak, N.N. Nikolsky, 2011, published in Tsitologiya, 2011, Vol. 53, No. 12, pp. 919–929.

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Zemelko, V.I., Grinchuk, T.M., Domnina, A.P. et al. Multipotent mesenchymal stem cells of desquamated endometrium: Isolation, characterization, and application as a feeder layer for maintenance of human embryonic stem cells. Cell Tiss. Biol. 6, 1–11 (2012). https://doi.org/10.1134/S1990519X12010129

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